Genome Organisation, Telomeres and Centromeres Flashcards

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1
Q

What is used to examine inter-molecular heterogeneity?

A

CsCl buoyant density gradients

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2
Q

Why is CsCl buoyant density gradients used?

A

Allow you to fractionate DNA based on relative GC content rather than charge or size

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3
Q

Where are most genes found?

A

In GC rich regions

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4
Q

G-band features

A

Heterochromatic - less transcription
Low GC rich content
Rich in L1 elements

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5
Q

R-band features

A

Euchromatic - transcriptionally active
High GC content
Rich in Alu elements

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6
Q

C-band features

A

Highly heterochromatic

Contain long arrays of satellite sequences

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7
Q

How much of the human genome is composed of transposable elements?

A

~45%

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8
Q

How much of the genome comprises of retrotransposons?

A

42%

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9
Q

What are the different types of transposons?

A

DNA based transposable elements
Autonomous retrotransposons
Non-autonomous retrotransposons

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10
Q

How much of the genome comprises of DNA based transposons?

A

~3%

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11
Q

What to DNA based transposable elements encode?

A

A transposase - enzyme that is responsible for cutting the element out of the genome and copying somewhere else

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12
Q

What are transposase homologous to?

A

Integrase genes

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13
Q

Are DNA based transposable elements active?

A

No

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14
Q

What are the two types of autonomous retrotransposons?

A

with long terminal repeats and without long terminal repeats

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15
Q

Example of a autonomous retrotransposon with an LTR

A

HERV (Human Endogenous RetroViruses)

Classes I, II, III

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16
Q

What 3 genes do the LTR autonomous retrotransposons contain?

A

Gag, Pol, Envelope

17
Q

Why can’t the LTR autonomous retrotransposon leave the cell?

A

Envelope is non-functional

18
Q

What is the most common type of non-LTR autonomous retrotransposon?

A

LINEs (Long interspersed nuclear elements)

19
Q

What is the most common type of LINE?

A

L1 elements