Genome organisation and gene expression

Question 1

What is the basic unit of chromatin?

Answer 1

The nucleosome

Question 2

What are the two sizes of main chromatin fibres?

Answer 2

10 nm and 30 nm

Question 3

What appearance do chromatin fibres have?

Answer 3

Beads on a string

Question 4

What technique is used to visualise chromosome territories?

Answer 4

FISH (Fluorescence in-situ hybridisation)

Question 5

Do chromosomes occupy distinct territories or are they randomly dispersed?

Answer 5

Distinct territories within the nucleus

Question 6

Gene-rich, highly transcribed, euchromatin-contained genes are found in the interior or the periphery of the nucleus?

Answer 6

Interior

Question 7

What is the region of the nuclear periphery where transmembrane proteins that attract heterochromatin are held?

Answer 7

Lamina

Question 8

What technique was used to test how peripheral recruitment affects gene expression?

Answer 8

Expressing an integrated reporter gene with a LacI/LacO inducer system