Genome Editing Using Crispr Flashcards
1
Q
How does the cas9 nuclease function.
A
The Cas9 nuclease together with a specific guide RNA locates the target DNA sequence that requires editing. Once in place, it make a precise double stranded cut. Once cut, the cellular material recognises the cut and gathers the machinery to repair the double stranded break. This is done through non-homologous end joining or homology- directed repair.
2
Q
How has cas9 been change for base editing.
A
Modified Cas9 protein makes single strand break and deaminase enzyme converts one base to another.
3
Q
How has cas9 been change for prime editing.
A
Modified Cas9 protein makes single strand break and with reverse transcriptase, can copy new sequences into nicked stand based on an extension of the guide RNA.
