Genome Annotation

Question 1

what is the important aspect of any genome sequencing project?

Answer 1

to make sense of the information along the flow of the genetic information (ie. DNA information transcribed into RNA information to be translated into proteins)

Question 2

what is FLYBASE

Answer 2

a database for Drosophila melanogaster and related species

Question 3

are there any point mutations mapped to our gene?

Answer 3

yes, there are a number of point mutations in our genes

Question 4

how are the genes expressed on the database

Answer 4

by drawing embryo genesis all the way to adulthood
- the peaks indicating expression of the various transcripts

Question 5

at does the J Browns tool do

Answer 5

tool on the database that assembles the information into a graphical interface

Question 6

where is the gene at the nucleotide level

Answer 6

have it set on “nucleotide view” to expand to see the individual nucleotides and potential open reading frames

Question 7

problems with the dataset

Answer 7

• data set is so large that you cannot see the actual data upon which your conclusions are based on

Question 8

what do the summaries show from the database

Answer 8

• summaries of what the protein encodes
• provides a description of the various pathways and protein functions
• a description of the types of alleles and constructs that have been made
• provides the genetics stocks that are available to order to carry

Question 9

describe the lishencephaly one gene

Answer 9

• protein coding gene
• encodes a regulator of a dining motor complex