Genome Flashcards
What are the three types of chromosomes in the tryp. genome?
11 Megabase, 5 intermediate and 100 mini
What sort of ploidy do they exhibit?
Aneiploidy= Variable numbers of chromosomes
Where do Trypanosomes bias towards GC?
At coding regions
Which genes are found in megabase chromosomes?
Housekeeping, Non expressed VSGs and PCUs
Which genes are found in intermediate chromosomes?
Surface Coat Protein
Which genes are found in mini chromosomes?
Additional Vsgs
What is a polycistronic transcription region?
Genes arranged in cluster on single strand and lack individual units
What does Aneiploidy provide?
Rapid environmental response but usually associated with severe abnormalities
What part of the genome is species specific?
The sub teleomeric regions
What did the genome release reveal?
Most VSGs are pseudogenes which m,ay be used to express mosaic genes.
Major differences in cytoskeleton
What does the ES serve to do?
Ensure transcriptional control over VSG
Facilitate antigenic switching
What is Rit-seq?
A method of high throughput phenotyping, mapping fitness costs associated with RNAi to genome sequence
Which species of Trypanosome lacks a midgut stage and why?
T. vivax and because it has a fundamentally different coat
Why can T. vivax be transmitted by other insects?
It has no procyclic stage
How has the Trypanosome genome been applied?
Drug Resistance- Escape mutations in Drug targets
Vaccine Design- Reverse vaccinology
Disease Diagnostics- VSG repertoire for population genetics
