Genetics, populations, evolution & ecosystem (3.7, chpt 17-19) Flashcards
Genotype
The genetic constitution of an organisms (the alleles it has for a gene)
Phenotype
The expression of the genes and its interaction with the environment
Homozygous
A pair of homologous chromosomes carrying the same alleles for a single gene
Heterozygous
A pair of homologous chromosomes carrying two different alleles for a single gene
Recessive allele
An allele only expressed if no dominant allele is present
Dominant allele
An allele that will always be expressed in the phenotype
Codominant
Both alleles are equally dominant and expressed in the phenotype
Multiple Alleles
More than two alleles for a single gene e.g. alleles for blood type
Sex-linkage
A gene who locus in on the X chromosome
Autosomal linkage
Genes that are located on the same chromosomes (not the sex-chromosomes)
Epistasis
When one gene modifies/masks the expression of a different gene at a different locus
Monohybrid
Genetic inheritance cross of a characteristics determined by one gene
Dihybrid
Genetic inheritance cross for a characteristic determined by two genes
How many alleles per gene do diploid organisms carry?
Two
Draw a Punnett square to show a monohybrid cross with parental genotypes of GG and gg
(pmt inheritance flashcards no.21)
Draw a Punnet square to show a monohybrid cross with parental genotypes of Gg and Gg
(inheritance flashcards pmt no.25)
Draw an example of a genetic diagram e.g. eye colour
PMT inheritance flashcards no.29
why do X and Y not form a bivalent (i.e. pair of homologous chromosomes)
as X and Y are different sizes so chromosomes are unable to line up, only short pairing regions
Give an examples of a characteristic that involves multiple alleles
Blood groups - IA, IB and IO
Why are actual ratios not exactly the same as theoretical ratios in genetics?
Because fertilisation of gametes occurs by chance - each time a gamete is fertilised, it is an independent event of what has gone before it.
Draw a Punnett square to show a dihybrid cross with parental genotypes of RrGg and RrGg
(inheritance flashcards pmt no.33)
Why are males more likely to express a recessive sex-linked allele?
- most sex-linked alleles located on x chromosome
- so males only get 1 copy of allele (so it’ll be expressed even if characteristic is recessive)
- since females get two alleles, this is less likely
Which parent do males inherit sex-linked characteristics from?
- their mother since the Y chromosome can only come from father
- so if mother is heterozygous for sex-linked alleles, she is a carrier and may pass on trait
Draw a Punnett square to show a sex-linked cross with parental genotypes of XHXh and XHY
inheritance pmt flashcards no.43
Define the two types of epistasis
recessive epistasis = where 2 homozygous recessive alleles mask expression of another allele
dominant epistasis = where 1 dominant allele masks expression of multiple other alleles
Draw a punnet square to show an epistasis cross with parental genotypes of AABB and aabb
pmt inheritance flashcards no.57
what is the chi-sqaured test?
- a stats tests to find out whether difference between observed and expected data is due to chance or a real effect
what are the criteria for the chi-sqaured test?
- data placed in discrete categories (as opposed to continuous data)
- large sample size
- only raw count data i.e. no percentages
- no data values equal zero
How is chi-squared test peformed?
- formula results in number
- number comapred to critical value (for corresponding degrees of freedom)
- if number greater than or equal to critical value we can conclude there’s a significant difference between observed and expected data and so results didn’t occur due to chance (in this case REJECT the null hypothesis)
Suppose you obtained a chi squared value of 5 and the critical value was 5.99 (2dof) - what can you conclude?
Because the chi squared value is SMALLER than the critical value - you accept the null hypothesis and there is greater than a 5% probability that the difference in the results are due to chance. There is no significant difference.
Suppose you obtained a chi squared value of 8 and the critical value was 5.99 (2dof) - what can you conclude?
Because the chi squared value is LARGER than the critical value - you reject the null hypothesis and there is less than a 5% probability that the difference in the results are due to chance. There is a significant difference.
how do you know which ‘degree of freedom’ to use in chi-sqaured?
number of categories - 1
chi-squared formula
x2= sum of: (O-E)2/ E
O= observed frequency E= expected frequency
what does more than two phenotypes indicate?
- codominance and/or multiple allele inheritnace
what six pieces of information must be shown in genetic crosses?
- parental phenotype
- parental genotype
- possible gametes
- offspring genotype
- offspring phenotype
- proportion of each phenotype (i.e. ratio)
when homozygous dominant is crossed with homozygous recessive what will be the genotype of the offspring?
- all heterozygous
unless there is autosomal linkage or crossing over in meiosis
In a dihybrid cross, if two heterozygous parents for both genes are crossed what will be the ratio you always get?
9:3:3:1
what is the impact of crossing over?
- new combinations of alleles in gametes
- therefore different proportions in punnet square
- however crossing over is rare
what is the impact of autosomal linkage?
- means alleles for each gene linked on the sane chromosome will be inherited together
- impacts the predicted gametes
what is the ratio of possible alleles of offspring when two heterozygous parents are genetically crossed with autosomal linkage?
3:1
when is it only possible to get all the phenotypes in autosomal linkage? and why would there only be few of these phenotypes?
- if crossing over occurred in meiosis to make new combinations of gametes
- crossing over is rare, so not many of these gametes formed by it will be made, therefore few of their genotypes formed and so few of the phenotypes expressed
if you don’t observe the ratio you expect in genetic crosses (e.g. monohybrid) why is that?
- crossing over has occurred
state types of inheritance
codominance multiple alleles sex-linkage autosomal linkage epistasis
Humans are diploid organisms. What does this mean? What about alleles does this explain?
We carry two copies of each chromosome - one from each parents.
What is the likely phenotypic ratio with a monohybrid cross of two heterozygous parents?
3 : 1
What things mean that you may not get the predicted phenotypic ratio?
codominant alleles and sex linkage
What would be the expected phenotypic ratio of a monohybrid cross between two heterozygous parents involving co-dominant alleles?
1 : 2 : 1
Give two reasons why the phenotypic ratio is not always the actual one.
Linkage or epistasis
What is a ‘carrier’ in terms of alleles
A person carrying an allele which is not expressed, but can be passed on to offspring
Why can’t males be the carriers of X-linked disorders?
they only have one X chromosome so if they have the disorder it will be expressed.
Why are genes on the same autosome said to be linked?
They stay together during independent segregation in meiosis 1, and their alleles will be passed on to offspring together
Why might the genes on the same autosome NOT be passed on together?
If cross over splits them up first
What is the relationship between the distance genes are from each other on the autosome and how closely they are said to be linked?
The closer they are on the autosome, the more closely they are said to be linked - crossing over is less likely to split them up
You are given the ratio 126 : 81 : 148 : 133. How would you simplify this ratio?
divide them all by the number on the far right to get a : b : c : 1
If the recessive allele is epistatic, how would it it mask the expression of the other gene’s allele?
You would need two copies of it
What is a null hypothesis?
It is that there is no significant different between the observed and expected results.
species exist as what?
one or more populations
Define Population
Group of organisms of the same species occupying a particular space at a particular time that can potentially interbreed