Genetics Flashcards
Operon
set of genes that are cotranscribed
Polysystronic mRNA
will be translated into several proteins
Genome:
entire complement of all genes in cell or virus
Chromosome:
main genetic element in Other genetic elements: virus genomes plasmids organellar genomes transposable elements
Plasmid
is a genetic element that is expendable and rarely contains genes for growth under all conditions
R plasmids
Resistance plasmids; confer resistance to antibiotics and other growth inhibitors
Widespread and well-studied group of plasmids
Many are conjugative
In what direction does replication occur?
5’ to 3’
Replisome
complex of multiple proteins involved in replication
DNA pulled through the replisome
Leading strand
has continuous synthesis
Lagging strand
has discontinuous synthesis
Okazaki fragment
short DNA fragments on lagging strand
DNA polymerase I
replaces RNA primer with DNA sequence
DNA ligase
covalently joins successive Okazaki fragments together
Codon:
a triplet of nucleic acid bases encodes a single amino acid
Chaperonins
They only assist in the folding, are not incorporated into protein
Can also aid in refolding partially denatured proteins
Extreme temperatures and pH