Genetics Flashcards
Prokaryotes
Bacteria and archaea
Lack a nucleus, one circular chromosome
No histones
70S ribosomes
No organelles
Peptidoglycan cell walls most bacteria (archaea cell walls are not PG)
Divide by binary fission
Eukaryotes
Fungi, protozoa, algae, helminths
True nucleus, several linear chromosomes
Histones
80S ribosomes
Organelles
Divide by mitosis
DNA in eukaryotes
In eukaryotes, linear DNA is found in long strands with associated proteins and is found in the nucleus. It’s wound around histone proteins. DNA and the histones are coiled to form chromosomes.
DNA in prokaryotes
In cytoplasm. Shorter and circular. Supercoiled to fit into the cell.
What is a gene?
A section of DNA which codes for polypeptides and RNA. There is a specific sequence of bases which code for particular polypeptides and RNA. Genes are found at a locus.
Alleles
Diff versions of the same gene
Homologous pairs
Pairs of chromosomes which contain the same genes at same locus but different alleles.
A degenerate code
Multiple codons can code for the same amino acid
Genome
The complete set of genes in a cell.
Proteome
The full range of proteins which a cell is capable of producing.
What are exons?
Sections of DNA which code for amino acids.
What are introns?
Sections which do not code for amino acids.
Does prokaryote DNA have introns?
No
Non-coding multiple repeats
Areas between genes in the genome that repeat over and over. Used in DNA fingerprinting.
What is mRNA?
A copy of a gene your body wants to make a protein for. Made during transcription and taken to ribosomes to be decoded and make proteins. In mRNA 3 bases adjacent to eachother is called a triplet.
Where is transcription in prokaryotes?
Cytoplasm
Where is transcription in eukaryotes?
Nucleus
Transcription
DNA helicase breaks h bonds between bases in DNA and causing it to unwind and separate into 2 strands and 1 strand acts as a template. RNA nucleotides attract to exposed bases by complementary base pairing.RNA polymerase joins RNA nucleotides together. Pre-mRNA spliced.
What begins transcription?
Promoter sequence
What ends transcription?
Terminator sequence
What is pre-mRNA?
MRNA containing introns and exons.
What is splicing?
Introns removed and exons are joined making mRNA. Takes place in nucleus.
Process of translation
MRNA attaches to ribosomes
TRNA anticodons bind to complementary mRNA codons
TRNA brings a specific amino acid
Amino acids join by peptide bonds with use of ATP
TRNA released
Ribosomes moves alone mRNA to form polypeptide
What is genetic code?
DNA is made of a code which can be read by ribosomes to build polypeptides. Ribosome reads a triplet at the same time. Base triplets do not share bases so the code is non-overlapping.
What is a diploid?
2n.
All cells in the body except gametes. Cells have chromosome pairs. Each pairs are homologous pairs.
Haploid
Only contains one of each of the homologous pair.
Meiosis
Type of cell division in eukaryotes that produces gametes. Cells start as a diploid to become a haploid.
Prophase 1 (1st division)
Each DNA strand shortens and becomes a condensed chromosome. Is visible. 2n
Prophase 1 continued
Each chromosome makes a copy of itself joined at the centromere.
Metaphase 1
Pairs of chromosomes line along the middle of the cell.
Anaphase 1
The pairs of chromosomes separate to opposite sides of the cell.