Genetic techniques Flashcards
1
Q
ChiP-seq
A
ChIP-chip methods: proteins are cross-linked to their bound DNA by formaldehyde treatment, cells are homogenized, and chromatin is sheared and immunoprecipitated with antibody-bound magnetic beads. The immunoprecipitated DNA is then used as the input for a next-generation sequencing library prep protocol, where it is sequenced and analysed for DNA binding sites.
2
Q
ATAC-seq
A
ATAC-seq captures open chromatin sites using a simple two-step protocol with 500–50,000 cells and reveals the interplay between genomic locations of open chromatin, DNA-binding proteins, individual nucleosomes and chromatin compaction at nucleotide resolution.
