Genetic lab techniques Flashcards
What are the 3 steps of PCR
- Denaturation
- Annealing - cooling, add primers
- Elongation - DNA pol replicates following primers
What are the two factors pulling protein in electrophoresis
Charge and Size
What happens in a Southern blot
Take a DNA sample (from electrophoresis gel) and use a labeled known DNA probe that anneals to complementary strand
What happens in a Northern blot
Take an RNA sample and use a DNA probe to find complementary strand
What is the sample in a Western blot
Protein
What is the probe in a Western blot
Labeled Antibody
What happens in a Southwestern blot?
DNA-binding proteins are the sample; The probe are labeled oligonucleotides.
What is the indirect ELISA
use a known test antigen to see if immune system recognizes it (if a specific antibody is present in the blood)
What is the “known” in an indirect ELISA
Antigen
What is the direct ELISA
Uses a known test antibody to see if a specific antigen is present in the patient’s blood
What is the “known” in a direct ELISA
Antibody
If there is a positive ELISA in an HIV test what is the confirmatory test?
Western blot
What is the probe in FISH
Fluorescent DNA or RNA
What does fluorescence indicate in FISH
If gene is present
What type of test uses a known antigen to discern the presence of an antibody
ELISA test
