Genetic Engineering

Question 1

Definition of Inbred

Answer 1

Strain established by a minimum of 20 consecutive

generations of sister × brother matings (sib matings)

Question 2

Definition of Outbred

Answer 2

Stock/line established and maintained by strict

avoidance of sib mating

Question 3

F1 hybrid

Answer 3

Created by mating mice of two inbred strains in a

defined direction

Question 4

F2 hybrid

Answer 4

Created by mating two F1 hybrid mice

Question 5

Recombinant

inbred

Answer 5

Lines established by inbreeding of the progeny of
individual F2 hybrid sibling pairs for at least 20
generations

Question 6

Congenic

Answer 6

Introduction of a mutation onto a defined inbred
strain background by 5–10 or more generations of
matings back to the inbred strain with selection for
the mutation (backcrossing)

Question 7

Consomic

Answer 7

An inbred strain that carries one chromosome from
another inbred strain
Ten generations of backcrossing and selection for the
donor chromosome is required for a fully consomic
strain

Question 8

Conplastic

Answer 8

An inbred strain that carries the nuclear genome of
one inbred strain and the cytoplasm and cytoplasmic
(mitochondrial) genome of another
Since the egg contributes the embryonic cytoplasm,
10 generations of backcrossing inbred females to an
inbred male recipient is required for a fully conplastic
strain

Question 9

RFLP

Answer 9

Restriction fragment length polymorphism - DNA is isolated, digested by bacterial restriction enzymes, then the lengths of the digested fragments compared for differences.

Question 10

SNPs

Answer 10

Single Nucleotide Polymorphisms - identified variations in single nucleotides used to monitor genotypes