Genetic Engineering Flashcards
GMOs
genetically modified organisms - an organism that have had its genome altered in some way
TGOs
transgenic organisms - organism that has had genes from another species inserted into its genome
advantages of viruses as vectors
viruses are well known for their ability to insert dna into a host cell. offer a chance for long-term stabilty, different viruses intergrate their dna into the host in different ways - scientists can control where the new dna is expressed in the host
disadvantages of viruses as vectors
size of dna is limited to 8000bps/8kb, intergration of the dna into the host dna can cause unexpected side effects depending on where in the host’s chromosome the dna inserts itself
advantages of plasmids as vectors
plasmids are circular lengths of dna that can be up to 1000kbs, they are frequently used, espeically in bacteria, the bacteria may be the final target for the recombinant dna or it can be used as vector to transfer dna to a different host
how are transgenic plants produced
a gene of interest is identifed and isolated, the isolated gene is then delivered into the cells of the host, either by direct insertion into the genome or use of a bacterial plasmid, the transformed cell is then grown repeatedly using plant tissue cultures
what are some applications of genetically modified plants
extending the shelf life of fresh produce, engineering plants to have pest or herbicide resistance, crop improvement (drought resistant crops or plants with higher protein levels)
how have soybeans been genetically modified
the have been modifed to produce more fat and vitamins for use as animal feed
how has pawpaw been genetically modified
modified to be resistant to papaya ringspot virus
how was golden rice a milestone in genetic engineering
scientists, for the first time, used a new metabolic pathway by splicing nre genes into an organisms dna. scientists switched onn a pathway that enabled the rice to produce and store β-carotene in the rice grain, ratther than just the leaves, meaning people would consume more vitamin a
how has bt corn been genetically modified
to contain the gene that produces the Bt protein which is a toxin that kills butterflies and moths but not affect other animals
benefits of genetic modification
increase crop yields (more nutrition, longer storage life), decrease use of pesticides, herbicides and animal remedies, drought/salt tolerant crops, improved human health and medicines
risks of genetic modification
possible uncontrollable spread of transgenes, GMO’s into environment may be irreversable, animal welfare and ethical issues, pest , insect or microbial resistance,
gmos and arent tgos
any organism that has had its has been genetically altered without having species dna inserted into them