genetic control of metabolism 2.7 Flashcards
wild strains of microorganisms can be made mire useful by
recombiant dna technology
mutagenesis
exposure to what may make an improved microorganism
uv light
radiation
mutagenic chemicals
what does rec dna tech invelve
rec plasmids
artificial chromosomes as genetic vectors
what is a genetic vector
a dna molecule used to carry foireign genetic info into another cell
examples of vectors
plasmids
artificial chromosomes
why are artificial chromosomes preferable to plasmids
they can carry more dna
what is the role of restriction endonucliase
to cut open the plasmids as vectors when foiregn dna is to be inserted
what are complimentary sticky ends
when the same restriction endonucliase cuts both the plasmid and gene from chromosome
what enzyme seals the gene in plasmid
ligase
what do recombinant plasmids and artificial chromosomes contain
restriction site
regulatory sequences
origin of replication
selectable markers
whats the role of restriction sites
they contain the section of dna needed to be transferred that the endonucliase cut
what are regulatory sequences
control the gene expression
role of origin of replication
allows self replication of the plasmod or artificial chromosome
role of selectable markers
antibiotic resistence gene
protect the microorganisms from a selective agent like antibiotic that would prevent growth or kill it
what do selectable markers do
make sure only those who have picked up the gene live
