Genetic Code and Transcription

Question 1

What are the features of the genetic code?

Answer 1

Unambigious (codon to one amino acid), degenerate (one amino acid to multiple codons), commaless, nonoverlapping, nearly universal

Question 2

Describe the wobble hypothesis

Answer 2

Codon anticodon bonding at third position is less constrained

Question 3

What is involved in prokaryotic transcription?

Answer 3

RNA polymerase, DNA template, promoter, sigma subunit that recognizes promoter, consensus sequences within promoter at -35 and -10, +1 transcription start site

Question 4

What are some features of prokaryotic transcription

Answer 4

Hairpin formation terminates transcription, transcription coupled to translation, polycistrionic mrna

Question 5

What is required for eukaryotic transcription?

Answer 5

RNA polymerase II, core promoter elements including the TATA box, which is analogous to the -10 sigma region in prokaryotes, general transcription factors, enhancers

Question 6

What are some features of eukaryotic transcription

Answer 6

occurs in nucleus, not coupled to translation, requires chromatin remodeling, introns spliced, postranscriptional proccessing