General Quick Knowledge

Question 1

Name the purine and pyrimidines

Answer 1

purines: adenine, guanine
pyrimidines: thymine, guanine, uracil

Question 2

list the hydrophobic amino acids

Answer 2

valine, leucine, isoleucine, methionine, proline

Question 3

name the aromatic amino acids

Answer 3

phenlyalanine, tyrosine, tryptophan

Question 4

name the amide amino acids

Answer 4

asparagine, glutamine

Question 5

name the basic amino acids

Answer 5

histidine, lysine, arginine

Question 6

name the nucleophilic amino acids

Answer 6

serine, threonine, cysteine

Question 7

what is the average molecular weight of an amino acid

Answer 7

110 Da

Question 8

what does the michaelis menten equation describe

Answer 8

the rate of enzyme catalysis at a particular substrate concentration

Question 9

what is the michaelis menten equation

Answer 9

v0 = Vmax [S]/ Km[S]

Question 10

list the size of these genomes in base pairs
human
mouse
zebrafish
fly
worm
yeast

Answer 10

3200 x 10^6
2800 x 10^6
1400 x 10^6
200 x 10^6
130 x 10^6
13 x 10^6

Question 11

define intron
define exon

Answer 11

sequences of DNA that are normally cut out of the final mRNA transcripts
DNA sequences that are kept in the final mRNA sequence

Question 12

central dogma

Answer 12

dna — rna — protein

Question 13

What are the steps to starting replication?

Answer 13

ORC get recruited to the origin of replication
ORC binding recruits CDT1 which recruits MCM2-7 helicase
More factors recruited to create pre initiation complex
PCNA holds polymerase onto the DNA and helicase and polymerase get phosphorylated and open up DNA

Question 14

define forward genetics

Answer 14

induce unbiased mutations and screen for mutant

Question 15

define reverse genetics

Answer 15

knock down every known gene and look for a phenotype