General Properties Of Antigen Antibody Reactions

Question 1

Union of Antigen and antibody requires formation of which bond?

Answer 1

Non covalent bonds:
Hydrogen bonds
Electrostatic interactions 
Hydrophobic interactions 
Van der waals forces

Question 2

Sum total of non covalent interactions between a single epitome of an Antigen with its corresponding paratope present on antibody.

Answer 2

Afinity

Question 2

Sum total of non covalent interactions between a single epitome of an Antigen with its corresponding paratope present on antibody.

Answer 2

Afinity

Question 3

The affinities of all the binding sites when multivalent antibody reacts with a complex Antigen carrying multiple epitopes.

Answer 3

Avidity

Question 4

Why are there less optimal binding interactions in multivalent antibody

Answer 4

Because it’s geometry gets stretched out to accommodate a complex antigens all epitopes

Question 5

Which is a better indicator of strength of an Antigen antibody reaction

Answer 5

Avidity

Question 6

Acidity increases with

Answer 6

Time

Question 7

Diagnostic tests based on ag ab reactions are called

Answer 7

Immunoassays also called serological tests as they are performed using serum samples

Question 8

Highest dilution of serum that shows an observable reaction with the antigen

Answer 8

Antibody titer

Question 9

False negative tests can be ruled out by

Answer 9

Quantitative assays

Question 10

True negative and false negative results can be differentiated by

Answer 10

Quantitative tests

Question 11

In marracks lattice hypothesis, ag ab reaction occurs at its best at

Answer 11

Middle test tubes

Or zone of equivalence

Question 12

In earlier test tube, ab are excess and in later test tube ag are excess , reactions do not occur in both, these are called

Answer 12

Prozone phenomenon and postzone phenomenon

Question 13

Ability of a test to identify correctly all those who have the disease I.e.
True positives

Answer 13

Sensitivity

Question 14

Formula of sensitivity a

Answer 14

True positive/True positives +false negatives

Question 15

Ability of a test to identify correctly all those who do not have the disease

Answer 15

Specificity

Question 16

Formula of specificity

Answer 16

True negatives /true negatives + false positives

Question 17

ELISA is an immunoassay that detects ag or ab in specimen by using

Answer 17

Enzyme substrate chromogen system for detection

Question 18

Two components of Elisa are

Answer 18

Immunosorbent

Enzyme

Question 19

Absorbing material used for elisa

Answer 19

Polystyrene

Polyvinyl

Question 20

Intensity of the color in Elisa reader is directly proportional to

Answer 20

The amount of detection molecule is ab or ag present in test serum