Gene Regulation Flashcards
At what 7 levels is gene expression regulated?
- transcription
- RNA processing
- RNA degradation
- translation
- protein processing
- protein degradation
- protein trafficking
What are polycistronic genes?
- genes that are expressed together in response to same signals
Where is TATA box in eukaryotes?
-30 bp
What is significance of Inr seq and where is it located?
- where DNA unwinds for transc
- @ -10 bp
T/F: in mammalian cell, proximal promoter can lack TATA box and Inr seq and still undergo transc
True
How do protein factors facilitate assembly/activation of PIC to start transc? (2)
- remodel DNA into a more active structure
2. bend DNA to interact w/ mediator
Function of transactivating domains?
- transc activators interact w/ chromatin remodeling proteins thru these domains
Function of HMG proteins?
- bend promoter DNA
Mechanism of repressor factors? (3)
- bind to upstream activating seq’s (UAS)
- prevents interaction b/w promoter and mediator
- inhibits mediator from facilitating PIC formation
Function of TATA binding protein (TBP)?
- orients PIC and ID’s strand usage
Functions of TFIIH? (2)
- helicase
- serine kinase subunit that phosphorylates CTD of RNA Pol II to activate PIC and transc
Mechanism of Wilms tumor gene (WT1)? (2)
- WT1 is a transc repressor important for kidney/gonad dvp’ment
- binds proximal promoter region to block transc of amphiregulin (EGF) and BCL-2 (anti-apoptosis) proto-oncogenes
Effect of loss of function mutation of WT1? (2)
- inappropriate oncogenic expression
- dvp’ment of solid kidney tumor in young kids
What is Wilms tumor linked to? (2)
- mesothelioma
- certain leukemias
Pit-1 transc factor function? (2)
- activates expression of GH and prolactin
- req’d for normal pituitary dvp’ment and hormone expression
