Gene organisation and transcription I

Question 1

Which strand of DNA is transcribed?

Answer 1

Anti-sense (it is anti-sense to the mRNA produced)

Question 2

What are the roles of the three types of RNA polymerase?

Answer 2

I – transcribes rRNA genes
II – transcribes genes encoding proteins into mRNA
III – transcribes tRNA and 5S RNA genes

Question 3

What is the role of gene promoter?

Answer 3

Transcription complex assembles at the gene promoter

Question 4

What constitutes the basal transcription complex?

Answer 4

TFII (D, A, B, F, E, H, J) and RNA polymerase II

Question 5

How do transcription factors affect gene expression?

Answer 5

By modifying histones Hyperacetylation = gene expression Hypoacetylation = gene repression