Gene expression III

Question 1

5 ways sequence specific DNA binding proteins are regulated

Answer 1

1. conformation of DNA binding protein altered by ligand binding
2. regulation of nucleus entry
3. regulation amount of transcription factors in cells
4. regulation of DNA binding
5. Protein degredation, recruitment of co-activatiors, and DNA binding altered by phosphorylation of DNA binding protein

Question 2

examples of ligand binding effects

Answer 2

1. zinc fingers transcription factors bind to steroid hormones
2. estrogen receptor activation
3. glucocorticoid receptor activation
4. tamoxifen mech of action cancer chemotherapeutic agent