Gene expression control

Question 1

1) Tataless with GC box and Inr.?
2) Tataless with GC box only?

Answer 1

1) TF II D only need TAF 1,2 to bind promoter
2) TF II D binds promoter via Sp1 and TAF 1,2,4 bind TBP to Sp1

Question 2

1) 2 subunits of TF II F?
2) What does TF II H do?
3) TF II S?

Answer 2

1) RAP 30, 70
2) phosphorylates CTD and melts DNA
3) Minimizes pause of RNA pol and stimulates elongation

Question 3

Classes of activators?

Answer 3

1) DNA-binding motifs:
- Zinc fingers
- bZIP
- Homeodomains
2) Transcription activating domains:
- Acidic domain
- Gln-rich
- Pro-rich

Question 4

Galactokinase expression…

Answer 4

no galactose: Gal 80p binds Gal 4p = blocks transcription
galactose: Gal 3p makes inducer that binds Gal 80p and moves it away from Gal 4p = activating transcription.

Question 5

Glucocorticoid receptor…

Answer 5

HSP 90 bound to receptor inactivates it
Glucocorticoid hormone binds to receptor and activates it