Gene expression Flashcards
Describe the major step of the transcriptional initiation in eukaryotic cells! What is the role of the general transcription factors in this process? Describe the roles of the transcription factors on gene expression regulation; in addition, describe the major kind of DNA binding domains of the transcription factors!
Describe the major step of the transcriptional initiation in eukaryotic cells! What is the role of the general transcription factors in this process? Describe the roles of the transcription factors on gene expression regulation; in addition, describe the major kind of DNA binding domains of the transcription factors!
Describe the structure of the nucleosome; compare the euchromatin with the heterochromatin! How does histone acetylation or methylation influence the gene expression? Which enzymes are responsible for the formation and elimination of these covalent modifications?
Describe the structure of the nucleosome; compare the euchromatin with the heterochromatin! How does histone acetylation or methylation influence the gene expression? Which enzymes are responsible for the formation and elimination of these covalent modifications?
Which enzymes are needed for the de novo DNA methylation? Which enzyme can maintain the DNA methylation pattern upon cell division? How DNA methylation is erased? How DNA methylation influence the chromatin structure and gene expression? How does the DNA methylation pattern change in advanced tumor cells?
Which enzymes are needed for the de novo DNA methylation? Which enzyme can maintain the DNA methylation pattern upon cell division? How DNA methylation is erased? How DNA methylation influence the chromatin structure and gene expression? How does the DNA methylation pattern change in advanced tumor cells?
Characterize the most important DNA regulatory regions (promoters, enhancers and insulators). Components of the nuclear hormone receptor systems. Compare the activation mechanism of the glucocorticoid receptor (GR) versus the retinoic acid receptor (RAR)!
Characterize the most important DNA regulatory regions (promoters, enhancers and insulators). Components of the nuclear hormone receptor systems. Compare the activation mechanism of the glucocorticoid receptor (GR) versus the retinoic acid receptor (RAR)!
Which transcription factors can be utilized for reprogramming of somatic cells into pluripotent stem cells? How can we introduce these factors to the somatic cells upon the iPS cell reprogramming? Characterize the viral vectors which are often used for ex vivo and in vivo gene therapies!
Which transcription factors can be utilized for reprogramming of somatic cells into pluripotent stem cells? How can we introduce these factors to the somatic cells upon the iPS cell reprogramming? Characterize the viral vectors which are often used for ex vivo and in vivo gene therapies!
