Gelatin Hydrolysis

Question 1

Do you think exprotease might be a constitutive or inducible enzyme , how could you test it

Answer 1

Filter bacteria off agar and put on nutrient gelatin, inducible

Question 2

Which exoprotease test is similar to the starch hydrolysis test

Answer 2

Gelatin

Question 3

What’s a commercial use for exoprotease

Answer 3

Leather, breaks down protien for chemical

Question 4

Would you expect exoprotease producers to be present in natural environments why?

Answer 4

Yes, decomposers break down dead organic material

Question 5

Are exoprotease members likely to be involved in association with other organisms

Answer 5

Yes, parasites. Intestine bacterial digestion

Question 6

How might exoprotease members be important medically

Answer 6

Remove dead tissues from wounds

Question 7

Why are the tubes cooled on ice after incubation

Answer 7

Solidify the gelatin

Question 8

Are there any special indicators in the medium

Answer 8

No

Question 9

Of what use would the hydrolysis of gelatin be to a cell

Answer 9

Nitrogen and carbon source, energy, dehydration protection

Question 10

What characteristics of agar make it more useful than gelatin for culture media

Answer 10

Solid above 22 degrees some bacteria can’t liquify it