Gel Electrophoresis Flashcards
1
Q
Why cut DNA?
A
Isolating the restriction enzymes are use to manipulate DNA
2
Q
Blunt ends
A
When both strands of DNA are cut directly opposite each other
3
Q
Sticky ends
A
When the second strand is not cut directly opposite the first cut
4
Q
Seperating DNA
A
Electrophoresis is used to sort out fragments into groups of their length. Copies are made by PCR (Polyerase Chain Reaction).
5
Q
Electrophoresis
A
1) DNA is isolated from cells
2) DNA cut into fragments
3) Fragments loaded onto gel
4) Electric field applied
6
Q
Electrophoresis electrical charges
A
DNA fragments have a net negative charge. They move to the positive poll. Shortest fragments move the quickest
7
Q
Cuting DNA into fragments
A
Restriction Enzymes. The double stranded DNA at a restriction site
