Gel Electrophoresis

Question 1

What is the function of the following:

• Buffer
• Power Supply
• Loading Dye

Answer 1

• Buffer: Ensures electrical conductivity.
• Power Supply: Applies a charged field with a positive and negative terminal.
• Loading Dye: Monitors the progress of DNA migration.

Question 2

Ethidium bromide is a toxic substance. Define its functions and dangers, as well as an alternative.

Answer 2

• To visualize separated DNA under UV light.
• Mutagen and toxic.
• Replace with methylene blue.