Gel electrophoresis (11 & 12)

Question 1

Southern

Answer 1

Target: DNA. Probe: ssDNA or RNA.

Question 2

Northern

Answer 2

Target: RNA. Probe: ssDNA or RNA.

Question 3

Western

Answer 3

Target: protein. Probe: antibody.

Question 4

Southern blotting uses

Answer 4

DNA fingerprinting, gene identification, gene cloning, genome mapping, chromosome walking, VNTR, RFLP

Question 5

DNA microarrays

Answer 5

1. RNA isolation.
2. cDNA generation.
3. Labelling of probe
4. Hybridised to array
5. Imaging & analysis