Gel Elctrophoresis

Question 1

Why would shorter ends migrate further in the gel?

Answer 1

Short stands move though the holes because they are smaller and fit

Question 2

Why is a buffer necessary when preparing the gel?

Answer 2

Salt water solution that will let the electrical charges flow through the gel

Question 3

Loading buffer?

Answer 3

Contains dye that makes the sample easy to see and makes the sample thicker

Question 4

Why does DNA have a negative charge? Why is a electric charge put through it in electrophoresis

Answer 4

Phosphate groups on the nucleotides have negative charge making DNA negative
-negative charge is put near he DNA which repels the DNA to the positive end of the sample

Question 5

Why is ethidium bromide used to treat the resultant gel?

Answer 5

DNA staining solution binds to the DNA and shows up under the fluorescent light

Question 6

Is gel electrophoresis used solely for analyzing DNA?

Answer 6

Used also for separating molecule like proteins