GC

Question 1

When to use GC

Answer 1

For volitile cpds

Question 2

Component of GC

Answer 2

-long columns: * packed columns: short length, long diameter
* capillary columns: long length , short diameter

Question 3

Why do we place columns in the temperature

Answer 3

So that the uniform temperature can be maintained

Question 4

Stationary phase

Answer 4

Made of silicone grease which can stand higher temperature

Question 5

Mobile phase

Answer 5

Made of gas(H or N) connected to the molecular sieve which remove unwanted water or oxygen for better separation

Question 6

What does elution depends on

Answer 6

More volatile molecules interact more with s.phase and elute first while less volatile elute later

Question 7

Detector used

Answer 7

FID flame ionisation detector

Question 8

How does FID work

Answer 8

Is made up of 3 inlet sample, oxygen and hydrogen, but it igniter ignites the O and N and produce a flame so when the sample molecules reach the flame they get ionised and electrons are released. Electrons are amplified by the detector which give the peak According to the retention of the sample

Question 9

Why do we need long tune or column

Answer 9

Hype long the column the better separation