GAPs Flashcards
cytokeratin 18
a cytoskeletal protein and the main intermediate filament family member expressed in the liver. Also used to identify Goblet cells within the intestinal epithelial layer
GP2
glycopprotein 2 - M cell marker
GAPs in GF and IL-10-/- mice
Both goblet cells and GAPs were more frequent in GF mice, and less frequent in IL-10-/- mice.
size exclusion for GAPs at steady state
Approx. 70kDa. beads from 0.02 to 1.0 um are excluded.
Math1
a transcription factor required for the development of secretory cell lineages, including GCs
Math1fl/flVilCre
mice lacking Math1 in cells expressing Villin-1 (This gene encodes a member of a family of calcium-regulated actin-binding proteins in the SI). These mice are devoid of goblet cells in the SI
GP-2
Glycoprotein-2, expressed only on M cells in the SI and shown to be recognized and bound to by FimH
Claudin-2
tight-junction protein that is associated with increased epithelial permeability and is increased in patients with Crohn’s Disease and Ulcerative Colitis
osteoprotegerin
-secreted decoy receptor of RANKL.
RegIIIg
C-type lectin produced by Paneth cells in the SI that is effective at preventing epithelial contact by Gram-positive bacteria
TFF3
Trefoil factor 3 - provides structural integrity to mucus through mucin crosslinking and acts as a signal that promotes epithelial repair, migration of IECs, and resistance to apoptosis
only terminally differentiated IEC that does not travel up the crypt-villi axis
Paneth cells, so that they can secrete AMPs to keep the crypts sterile-ish
What does NOD2 recognize?
Muramyl dipeptide (MDP) -
Chemokine receptor for monocyte recruitment to the intestine
CCR2
murine cDC1 transcription factors
interferon regulatory factor 8 (IRF8), basic leucine rich zipper transcription factor ATF-like 3 (BATF3), and inhibitor of DNA-binding-2 (ID2)
