G2 Flashcards
1
Q
What does histological technique deal with?
A
The preparation of tissue for microscopic examination.
2
Q
What is the aim of good histological technique?
A
To preserve the microscopic anatomy of tissue.
3
Q
What is the thickness of sections that can be made with good histological technique?
A
Very thin sections of 4 to 5 microns.
4
Q
What are the two types of specimens in histology?
A
- Biopsy: piece of tissue or organ taken from a living human being.
- Autopsy: piece of tissue or organ taken from a dead body.
5
Q
What are examples of fluids studied in histology?
A
Blood, cerebrospinal fluid (CSF), ascites fluid, pleural effusion fluid.
6
Q
What are the steps to study a specimen?
A
- Gross examination: colour, size, surface, texture, consistency.
- Microscopical examination: examine under microscope.
7
Q
What are the steps in tissue preparation?
A
- Fixation
- Dehydration
- Cleaning
- Embedding
- Cutting
- Staining
