Functional studies/ Zebrafish

Question 1

Why do we need animals in research?

Answer 1

Cells behave differently in vitro to in vivo

Question 2

Number of disease causing genes in common with humans (%) is higher is zebrafish compared to mice.

True or false

Answer 2

False, it is smaller

Question 3

The Number of genes (protein coding) genes in zebrafish is higher than that compared to humans, mice and fruitfly.

True or false

Answer 3

True

Question 4

Why are zebrafish used?

Answer 4

Cheap to maintain and easy to breed.

Rapid Ex-utero and virtually transparent development.

Lots of eggs

Vertebrates with similar genetics to humans.

Heart development is similar to higher vertebrates

Question 5

Unlike mammals, which havefour chambers, the zebrafish heart consists of what…

Answer 5

Only two: a single ventricle (left) and a single atrium (right).

Question 6

Briefly explain the life cycle of zebrafish

Answer 6

30 minutes after fertilisation

Cleavage begins

Gastrulation and epiboly

Organogenesis

Hatching

Adult

Question 7

What is in situ hybridisation used for?

Answer 7

Gene expression

Question 8

What technique uses antibodies to localise proteins

Answer 8

Fluorescent Immunohistochemistry