functional genomics Flashcards
1
Q
what is meant by the term ‘transcriptome’?
A
the sum total of all gene expression activity in the cell
2
Q
which techniques can be used to analyse the transcriptome?
A
- cDNA sequencing
- EST library sequencing
- SAGE: serial analysis of gene expression
- tissue specific cDNA macroarrays
- microarrays
- RNA seq
3
Q
what is the advantage of sequencing based techniques versus hybridisation based techniques?
A
sequencing based techniques can support gene prediction
hybridisation based techniques require pre-existing annotation - can’t be used for predictions
4
Q
what is the proteome?
A
all the proteins in the cell
5
Q
how can proteins of indentical mass be separated?
A
they usually have different isoelectric points and therefore can be separated using 2D gel electrophoresis
6
Q
what is meant by interactive?
A
the complexes that interact in the cell
