fuck

Question 1

troubleshooting issues 3 ways

Answer 1

with instrumentation

such as wear and tear on parts
00:44
contamination in the system for example
00:46
we can look at the LC troubleshooting
00:48
from the standpoint of columns is the
00:50
column the right one for the sample at
00:52
hand in the case of gradients has it
00:54
been equilibrated correctly and we can
00:57
look at troubleshooting from method
00:58
development viewpoint are your
01:00
conditions correct have you modified
01:02
your flow rate to account for a change
01:04
in column dimension

Question 2

troubleshooting is un

Answer 2

so forth when it
01:07
comes to troubleshooting 90% of the
01:10
issue comes down to understanding how to
01:12
identify where the issue is coming from
01:15
attaching a symptom to the actual source
01:18
often we get chromatographer x' who call
01:22
with a symptom and they want us to help
01:25
with a solution my peak shapes are
01:28
terrible for example our technical
01:31
support people can only begin to help
01:34
them by asking a lot of questions
01:36
designed to help exclude potential
01:39
sources and understand enough to be able
01:43
to determine whether they need to be
01:45
thinking about the instrument the column
01:48
or the method

Question 3

system map at noramco

Answer 3

what is normal?

Question 4

shane excel

Answer 4

gpv

Question 5

when micron sized particles

Answer 5

micron sized particles are packed into a
00:35
column and liquid is pushed through
00:37
these particles by your pump there's a
00:39
lot of resistance it's this resistance
00:41
to flow that causes back pressure

Question 6

what affects column back pressure:

Answer 6

1. Solvent back pressure
2. Flow rate
3. climb length
4. particle diameter
   he particle size is reduced by one-half 02:38pressure rises by a factor of four
5. column diameter

Question 7

1. 
increasing a lower
2. 03:37
pressure is most often result of a
3. 03:39
system leak or a loss in pom Prime the

Answer 7

system leak or a loss in pom Prime the

Question 8

third area is fluctuating pressure or
* 03:45
constantly changing pressure fluctuating
* 03:47

Answer 8

1. pressure is often a result of check
   03:50
   valve sticking or malfunctioning since