Frozen Tissue Sections

Question 1

Common Principle Involved in Frozen Sections

Answer 1

Process which tissue sections or blocks are undergo RAPID PRESERVATION of tissue block by FREZZING/QUENCHING for instant cessation of cellular activities thereby preventing chemical alteration of tissue components.

Question 2

Methods employed in Frozen Section

Answer 2

Freeze Frozen Tissue Method
Freeze Drying Method
Freeze Substitution Method

Question 3

Most common Freezing agent

Answer 3

Liquid Nitrogen

Question 4

This involves a physical process “DESICCATION” between -30C to -40C within 24-48 hrs

Answer 4

Freeze Drying Method

Question 5

Freeze Substitution

Answer 5

Process of simultaneously dehydrating and

chemically fixing a sample and by slowly increasing the temperature using a freeze substitution equipment.

Question 6

Freeze Substitution fluids

Answer 6

Rossman’s fluid

Osmium Tetroxide in 1% acetone

Question 7

Histopath Reports must be prepared in how many copies?

Answer 7

3 copies

Question 8

Designated to whom the 3 copies will be given.
Original
Duplicate
Third Copy

Answer 8

Original Copy - Patients
Duplicate Copy - Physician
Third Copy - Hospital Records