Frozen Sections Flashcards
what should the temp of the cryobar/platform be
around -30 celsius to -50 celsius
explain the glass slide trick
chill a glass slide
place tissue directly on it with side to cut down
OCT on slide and chuck
place chuck on slide
warm finger over glass
why shouldn’t use leave a boarder of OCT around muscle
causes freeze artifact
how do you fix a section that shreds as it comes off the knife
new knife
temperature control
how do you fix a bunching section
clean blade holder with 100% ethanol
cool blade
adjust angle
move knife
how do you fix too thick/thin sections
check machine
lubricate instrument
what is thionin and how is it used
gives a rapid nuclear stain for frozen
fix a slide in 95% ethanol than place a thionin on it for 10 seconds
drain on paper towel and rinse with tap water
what is Oil Red O (ORO) stain used for
demonstrates neutral lipids in frozen sections
what mounting media should you use for muscle instead of OCT
tragacanth gum
what is a ATPase stain used for
differentiates between type 1, type 2A, and type 2B muscle fibers
what are the results of a ATP 4.3 stain
type 1 fibers: very dark
type 2A: light
type 2B: intermediate
what are the results of a ATPase 9.4 stain
type 1 fibers: lights/unstained
type 2 fibers: dark
