Fried lectures

Question 1

What is the term for any DNA monomer minus the phosphate group ?

Answer 1

2’deoxyribosyl nucleoside

Question 2

What effect does the extra OH have on the ribose ring compared with deoxyribose?

Answer 2

Stiffer!

Question 3

What form of ribose is in ADP, ATP?

Answer 3

Regular ribose ( both hydroxyls)

Question 4

What enzyme breaks down pyrophosphate?

Answer 4

phosphodiesterase

Question 5

What does G’0 indicate

Answer 5

The free enery at standard biological conditions; 1 equivalent, pH7, Water conc. at 55M, 25C temperature

Question 6

What are the 2 advantages of breaking an NTP

Answer 6

Charge separation, availability of resonance structures

Question 7

What is the dominant tautomer of Adenosine and Cytosine? The less prevalent form?

Answer 7

Amino=dominant, Imino= less prevalent

Question 8

What is the dominant tautomer of Thymine and Guanine? The less prevalent form?

Answer 8

Keto=dominant, Enol= less prevalent (one oxygen made hydroxide)

Question 9

What are the major properties of BDNA?

Answer 9

10.4 bp/turn, right-handed, major& minor grooves

Question 10

What are the major properties of A-DNA?

Answer 10

Right-handed, more broad&short than bDNA, deep&wide major groove

Question 11

What are the major properties of Z-DNA?

Answer 11

LEFT-handed, shallow&narrow grooves,

Question 12

Why is U not tolerated in DNA

Answer 12

C can be deaminated to make U