forensic dna markers Flashcards
1
Q
what are point mutations?
A
one or more bases affected
can be random or due to environmental factors
2
Q
what are polymorphisms
A
more than two forms exist
airse due to block or point mutation
passed onto offspring
3
Q
SNP
A
short nucleotide polymorphisms
4
Q
STR
A
short tandem repeats
5
Q
RFLP analysis
A
restriction
- restriction enzymes digest dna
fragment
- shorter fragments are present once DNA is digested
length
- determined by electrophoresis
polymorphisms
- the existence of more than one DNA marker
6
Q
stutter
A
biological artefact associated withpcr amplification of repetitive DNA
–> caused by polymerase slippage during replication
7
Q
what makes a good dna marker
A
polymorphic
low mutation rate
large number of alleles in human population
short sequence length
