Forensic DNA database Flashcards
1
Q
What is CODIS?
A
Combined DNA Index System
CODIS is the FBI’s program of support for criminal justice DNA databases as well as the software used to run these databases.
2
Q
How many core loci underly CODIS and how many population datasets are included?
A
13 core loci
over 50 population databases; more than 10 000 individuals
3
Q
What does YHRD stand for?
A
Y-STR Haplotype Reference Database
4
Q
How many core loci are defined by SWGDAM for Y-STR analysis?
A
11
5
Q
SWGDAM recommendations for mtDNA typing
A
- GLP and follow specific protocols
- Negative and positive controls and extraction reagent blanks
- reported sequences must be based on redundant information from forward and reverse primers
- manual transcription should be avoided
- independent confirmation by two scientists
- labs shall participate in proficiency testing programs
- sequence entire mitochondrial DNA control region in population genetic studies
- report relative to rCRS, allignment needed
- use IUPAC convention
- use analytical software that checks for phylogenetics
- be able to justify choice of database and statistical approach
- establish statistical guidelines for reporting match between two samples
6
Q
SWGDAM recommendations for mtDNA heteroplasmy
A
- labs have to establish their own guidelines on how do deal with them
- “differences in both PHP and LHP do not constitute evidence for excluding two otherwise identical haplotypes as deriving from the same source or same maternal lineage”
- put the dominant variant into database