Foodborne pathogens Flashcards
Food born infection
Infection is caused by ingestion of food that is contaminated with live pathogens such as bacteria, viruses, or parasites and they act directly on the intestines.
food borne infection examples
E.g. Salmonella spp.. Listeria monocytogenes, Vibrio parahaemolyticus, Campylobacter jejuni, Yersinia enterocolitica.
Food borne intoxication
Intoxication is caused by ingestion of food which is ALREADY CONTAMINATED with toxins produced by bacteria (pre-formed toxins)
example food born intoxication
E.g. Staphylococcus aureus, Clostridium botulinum
Foodborne toxico infection
Toxico-infection is caused by ingestion of food contaminated with live pathogens and they produce toxins inside the host.
The bacteria either sporulate (spore-formers) or die (cell lysis) (non spore-formers) and release toxins in the gastrointestinal tract (intestines).
example of food born toxico infection
E. coli 0157:H7, Bacillius cereus, Clostridium perfringens.
Gram positive
Clostridium botulinum
Clostridium perfringens
Bacillus cereus
Staphylococcus aureus
Listeria monocytogenes.
Gram negative
Salmonella
Campylobacter
Yersinia
Vibrio chalera
Vibrio parahaemolyticus
* Aeromonas hydrophila
Escherichia coli
Foodborne botulism
The botulinum toxin is heat labile and can be destroyed if heated at 80°C for 10 minutes or longer.
Optimum range for clostridium perfringes
(43°C-47°C)
Clostridium perfringes
Temperature abuse of prepared foods can cause food poisoning
The spores survive cooking and it germinate into vegetative cells as the food cools
Prevention of clostridium perfringes
Cook and keep food at the correct temperature keep food at ›60C or < 4C
Meat dishes should be served hot within 2 hours after cooking
Refrigerate leftovers
Reheat them properly (>74C) before serving
Bacillus cereus
Onset of watery diarrhea.
abdominal cramps - mimic the C. perfringens poisoning.
Bacillus cereus caused by? (diarrhea type
Caused by enterotoxin produced in intestine
Bacillus cereus caused by? (vomitting type
caused by emetic toxin produced
Staphylococcus aureus
produce heat-stable enterotoxin in food that causes staphylococcal food poisoning - intoxication
food implicated for staphylococcus aureus
-Bakery products such as cream-filled pastries. cream pies, and chocolate eclairs; sandwich fillings; and milk and dairy products.
-Foods that require considerable handling during preparation and that are kept at slightly elevated temperatures after preparation are frequently involved in staphylococcal food poisoning.
main source of contamination in RTE foods
Human intoxication is caused by ingesting enterotoxins produced in food by some strains of S. aureus, usually because the food has not been kept hot enough (60°C, 140°F, or above) or cold enough (7.2°C, 45°F, or below).
Listeria monocytogenes
cervical infections in pregnant women, which may result in spontaneous abortion (2nd/3rd trimester) or stillbirth
Listeria monocytogenes Usually preceded by
influenza-like symptoms including persistent fever.
species of Salmonella
S. enterica, S. bongori
Salmonellosis
nontyphoidal and typhoidal
Non-typhoidal
dehydration due to diarrhea and vomiting - may lead to death
-arthritis may follow 3-4 weeks after onset of acute symptom (e.g. joint inflammation)
Thyphoid fever caused by
S. Thyphi & S. parathyphi
Symptoms for typhoid fever
High fever, diarrhea, headache, drowsiness, abdominal pain, loss appetite sometimes rashes - 10% mortality
main groups of pathogenic E. coll
Enterotoxigenic E.coli (ETEC)
Enteropathogenic E. coli (EPEC)
Enterohaemorrhagic E. coli (EHEC)
Enteroinvasive E. coli (EIEC)
Campylobacter jejuni
The illness usually occurs 2-5 days after ingestion of the contaminated food or water.
Yersinia enterocolitica
Yersiniosis is frequently characterized by such symptoms as gastroenteritis with diarrhea and/or vomiting, fever, abdominal pain
Vibrio cholerae
Outbreak of cholerae is associated with the contaminated water, consumption of raw or undercooked shellfish
chlorosis is caused by
the ingestion of viable bacteria; which attach to the small intestine and produce cholera toxin - toxicoinfection
Vibrio parahemolyticus
Diarrhea, abdominal cramps, nausea, vomiting, headache, fever, and chills may be associated with infections caused by this organism.