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Food hygiene > Food hygiene midterm 1 > Flashcards

Food hygiene midterm 1 Flashcards

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1
Q 
Which statement is True for Salmonella?
A. Indol production is negative
B. Voges-Proskauer test is positive
C. Lacotse-Fermentation is positive
D. H2S production is positive
A

A. Indol production is negative

D. H2S production is positive

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2
Q

Which statement is False for Listeria monocytogenes?
A. Catalase test is positive
B. Rhamnose negative
C. Its typical colonies on ALOA agar are blue
D. Shows summer saulting motility at 25°C

A

B. Rhamnose negative

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3
Q 
Which statement is False for E.coli?
A. Citrate utilization test is positive
B. Indole test is positive
C. Voges-Proskauer test is negative
D. Methyl red test is positive
A

A. Citrate utilization test is positive

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4
Q 
Enterococcus faecalis can grow:
A. Between 10-60°C
B. At pH 2.6
C. At 6.5% salt concentration
D. Grow on blood agar with 70% bile content
A

C. At 6.5% salt concentration

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5
Q

Which statement if False?
A. Limulus test is suitable for detection of Gram-bacteria
B. Impedance measurement works with special media only
C. The microbial count can be determined by detecting the amount of ATP
D. The redox potential of the medium decreases during the bacterial growth

A

C. The microbial count can be determined by detecting the amount of ATP

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6
Q

Which statement is True?
A. Enterococcus faecalis is catalase positive
B. Staphylococcus aureus is cultured in Fraser broth
C. Positive colonies of E.coli are bluish green on TBX agar
D. Enterobacteriaceae cannot ferment glucose

A

C. Positive colonies of E.coli are bluish green on TBX agar

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7
Q

Which statement is False?
A. Listeria monocytogenes’ b-haemolysis strengthens the haemolysis of Staphylococcus aureus
B. Salmonella colonies are reddish purple on XLD agar
C. Salmonella decomposes lactose
D. MRSV agar can be used instead of RVS broth in the enrichment procedure of Salmonella

A

C. Salmonella decomposes lactose

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8
Q 
Which statement is True for Clostridium perfringens?
A. Cultured on Sulphite cycloserine agar
B. B and C strains pathogenic for humans
C. In LS broth incubated at 25°C
D. No haemolysis on blood agar
A

A. Cultured on Sulphite cycloserine agar

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9
Q

Which statement is False?
A. Selective media of moulds have low pH
B. Enterococcus faecalis is one of the most resistant among the non-spore forming microbes
C. Enterococcus faecalis is catalase positive
D. E.coli can ferment lactose

A

C. Enterococcus faecalis is catalase positive

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10
Q 
Which statement is True for Staphylococcus aureus?
A. Produces toxin between 8-45
B. Reduces the nitrate to nitrite
C. No haemolysis on blood agar
D. Gram negative
A

B. Reduces the nitrate to nitrite

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11
Q

What are the suitability of sampling based on suspicion and annual monitoring plan?
Write the letters to the adequate line:
A) It is used to inspect the cause of foodborne disease
B) It is used to determine the drug residues in the food
C) It is used to investigate the chemical contaminants of the food
D) It is used to detect the poisonous source

A

Sampling based on suspicion: A, D

Sampling based on monitoring plan: B, C

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12
Q 
Classify the different screening methods:
A) Meatsafe test
B) KIS test
C) Brilliant black reduction test
D) Snap test
A

Microbial growth inhibition test: KIS, Brilliant black reduction test – B, C
Receptor based tests: Snap-test, Meatsafe test – A, D

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13
Q

What is the quantity of one sample based on the legal regulation in case of the foods
below? Pair the sample with the adequate quantity:
A) Turkey meat
B) Stone fruit
C) Mammal fat
D) Cow milk
500ml, 500g, 1kg

A

500ml: Cow milk
500g: Turkey meat, Mammal fat
1kg: Stone fruit

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14
Q

What are characteristics to the objective inspection program and the selective control?
Write the letters to the adequate line
A. It is used to survey attributes of food
B. It is used to screen the non-compliant items occurring rarely
C. The sample is selected by targeted risk evaluation
D. The results of the investigation are valid for the whole population

A

Objective inspection program: A, D

Selective control: B, C

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15
Q

Whats true for E. coli?

A

Indole +

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16
Q

Whats true for Enterococcus?

A

facultative anaerobic

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17
Q

There are 5 samples. One of them is salmonella +. What to do?

A

Bann it!

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18
Q

Lysteria monocytogenes- which is true?

A

Ramnose+, xilose -, camp test + w/ staph aureus. This

was one answer

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19
Q

On which pH is the sorbine acid the most effective?

A

5.5-6.0

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20
Q

Whats true for staph aureus?

A

It has thermo-stabile desoxi ribonuclease

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21
Q

What is the meaning of TDT?

A

Thermal Death Time

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22
Q

Botulinum toxin

A

Neurotoxic

causes diarrhea

23
Q

Which is psycrophile?

A

Staph aur.

Yersinia enterocolitica

24
Q

Where does the toxine of clostridium perfringes generate?

A

In the intestinal system

In the food.

25
Q

Whats is the letter z stands for?

A

Z-value –> change in the temperature whic results in a tenfold change in the Decimal reduction time (D) or Thermal Death Time (TDT)

26
Q

Whats is the letter z stands for?

A

Z-value –> change in the temperature whic results in a tenfold change in the Decimal reduction time (D) or Thermal Death Time (TDT)

27
Q

There are 44 colonies on the agar. The dilution is 10^-4. Whats the number of the microbes?

A

N=44/ (1(1)10^-4) = 44/10^-4 = 4.4*10^5

28
Q

inoculation volume of pour plate and spread plate

A

Pour plate - 1 ml, spread plate - 0,1 ml

29
Q

Acid production of Listeria

A

L- monocytogenenes - ramnose +, xylose -

L. ivanovii - Ramnose -, xylose +

30
Q

Purpose of desoxycholate in XLD medium

A
  • inhibits Gram+

- inhibits Proteus swarming

31
Q

Biochemical reactions of Salmonella

A

+ Glucose decomposition with acid production
+ Glucose decomposition with acid and gas production
+ Lysine test
+ Methyl red test
+ H2S test

  • Indol test
  • Lactose fermentation
  • Sucrose fermentation
  • Urease test
  • Voges - Proskauer test
  • ONPG test
32
Q 
What is the quantity of one sample based on the legal regulation in case of foods below?
Pair the sample with the adequate quantity
A. Mammal meat
B. Pulse (leguminous)
C. Sausage
D. Milk
500ml: 
500g:
1kg:
A

500ml: Milk
500g: Mammal meat, Sausage
1kg: Pulse

33
Q

What is not true for gas chromatography?
A. It is not suitable for analyzing inorganic materials
B. Heat sensitive materials can also be analyzed
C. It has higher resolution capacities than liquid chromatography
D. It can be connected with mass spectrometric detection

A

C. It has higher resolution capacities than liquid chromatography

34
Q

What is the main bottleneck of molecular spectroscopy?
A. Detection and quantification levels are not low enough
B. It is not suitable for analyzing solid samples
C. Only one compound can give signal in the given wavelength
D. It requires too high amount of analytical sample

A

C. Only one compound can give signal in the given wavelength

35
Q

What are not characteristic of indicator organisms?
A. Should be informative in all types of waters? (drinking water, lake, river, seaweed, wastewater etc.)
B. They are obligate pathogens
C. Characterized by longer survival time than the general enteral pathogenic microbes
D. Their quantity must show direct correlation with the degree of fecal contamination

A

B. They are obligate pathogens

36
Q

Which statement is True for Pseudomonas aeruginosa?
A. Gram positive, rod shaped, oxidase positive, non-spore forming bacterium
B. Occurs in feces, soil, water and waste water
C. As an indicator of fecal contamination can be used
D. Provides information about defects of filtering process

A

B. Occurs in feces, soil, water and waste water

37
Q

Which statement is False for Sulphite reducing Clostridia and Clostridium perfringes?
A. Obligate anaerobic, spore forming bacteria
B. Most representative is Clostridium botulinum, which is natural member of fecal microflora
C. Spore can survive in the water for long time and are highly resistant to disinfection
D. Presence in drinking water indicates the possibility of protozoa cysts and defects of filtering process

A

B. Most representative is Clostridium botulinum, which is natural member of fecal microflora

38
Q

Which statement is False for heterotrophic aerobic bacteria?
A. Determined at 15°C and 37°C
B. Long term examination can be used for control the treatment of water
C. Sudden increase in the number refers to a defect in the water treatment
D. Carried out by pour plate method

A

A. Determined at 15°C and 37°C

39
Q

Which statement is True for total coliforms?
A. Associated with a more or less fecal contamination
B. Direct indicators of fecal contamination nor have health risk
C. Presence in drinking water indicates the possibility of protozoa cysts
D. Belonging to the Streptococcaceae family

A

A. Associated with a more or less fecal contamination

40
Q

Which statement is True for the organic acids as preservatives?
A. Usually these substances have bactericide effect
B. The organic acids are effective in dissociated form
C. The effectiveness of the preservatives depends on concentration temperature
D. At low pH value the degree of dissociation is higher

A

C. The effectiveness of the preservatives depends on concentration temperature

41
Q

Which statement is True for the water removal?
A. The microbes can multiply at each water activity circumstances
B. The reducing of water activity inhibits the multiplication of microorganisms
C. The bacteria can grow below 0.8 Aw
D. The Aw requirement of bacteria is lower than the moulds

A

B. The reducing of water activity inhibits the multiplication of microorganisms

42
Q

Which statement is True for the heat treatment?
A. All of the vegetative cells can be eliminated with 60c heat treatment
B. Enterococcus can survive the 80°C 30 min. heat treatment
C. All the spores can be eliminated with 80°C heat treatment
D. The most commonly used preservation method in the food industry

A

D. The most commonly used preservation method in the food industry

43
Q 
The typical spoilage flora of meat products are?
A. Moulds
B. Yeasts
C. Micrococci, Lactobacilli
D. Salmonella spp.
A

C. Micrococci, Lactobacilli

44
Q 
The typical spoilage flora of bakery products are?
A. Moulds
B. Lactobacilli
C. Yeasts
D. Clostridium botulinum
A

A. Moulds

45
Q

What are characteristic of indicator organisms?
A. Should be informative in all types of waters? (drinking water, lake, river, seaweed, wastewater etc.)
B. They are obligate pathogens
C. Characterized by shorter survival time than the general enteral pathogenic microbes
D. Their quantity does not show direct correlation with the degree of fecal contamination

A

A. Should be informative in all types of waters? (drinking water, lake, river, seaweed, wastewater etc.)

46
Q

Which statement is False for Pseudomonas aeruginosa?
A. Gram negative, rod shaped, oxidase positive, non-spore forming bacterium
B. Occurs in feces, soil, water and waste water
C. As an indicator of fecal contamination can be used
D. Provides information about the general cleanliness of the water distribution system

A

C. As an indicator of fecal contamination can be used

47
Q

Which statement is True for Sulphite reducing Clostrdia and Clostridium perfringes?
A. Obligate aerobic, spore forming bacteria
B. Most representative is clostridium botulinum, which is natural member of fecal microflora
C. Spore can survive in the water for long time and are highly resistant to disinfection
D. Provides information about the general cleanliness of the water distribution system

A

C. Spore can survive in the water for long time and are highly resistant to disinfection

48
Q

Which statement is True for heterotrophic aerobic bacteria?
A. Determined at 15°C and 37°C
B. Long term examination can be used for control the treatment of water
C. Sudden increase in the number refers to a fecal contamination in the water treatment
D. Carried out by MPN method

A

B. Long term examination can be used for control the treatment of water

49
Q

Which statement is False for total coliforms?
A. Associated with a more or less fecal contamination
B. Direct indicators of fecal contamination nor have health risk
C. Provide good information on the microbiological status of the water
D. Belonging to the Enterobacteriaceae family

A

B. Direct indicators of fecal contamination nor have health risk

50
Q

Which statement is False for the organic acids as preservatives?
A. Usually these substances have bacteriostatic effect
B. The organic acids are effective in undissociated form
C. The effectiveness of the preservatives depends on concentration temperature
D. At low pH value the degree of dissociation is higher

A

D. At low pH value the degree of dissociation is higher

51
Q

Which statement is False for the water removal?
A. The microbes can multiply at certain water activity circumstances
B. The reducing of water activity inhibits the multiplication of microorganisms
C. The bacteria can grow below 0.7 Aw
D. The Aw requirement of bacteria is higher than the moulds

A

D. The Aw requirement of bacteria is higher than the moulds???

52
Q

Which statement is False for the heat treatment?
A. All of the vegetative cells can be eliminated with 60°C heat treatment
B. Enterococcus can survive the 60°C 30 min. heat treatment
C. All the spores can not be eliminated with 80°C heat treatment
D. The most commonly used preservation method in the food industry

A

A. All of the vegetative cells can be eliminated with 60°C heat treatment

53
Q

What part of the electromagnetic radiation spectrum can be used for analytical purposes?
A. Only the ultraviolet and the visible parts
B. The ultraviolet, visible and near infrared parts
C. The ultraviolet, visible, infrared and the x-ray parts
D. The whole spectrum can be used

A

C. The ultraviolet, visible, infrared and the x-ray parts

54
Q

The main bottleneck of mass spectrometry?
A. Detection and quantification levels are not low enough
B. It is not suitable for analyzing solid samples
C. It is suitable only for structure analysis of chemically clean materials
D. It requires too high amount of analytical sample

A

C. It is suitable only for structure analysis of chemically clean materials

Food hygiene (1 decks)

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