Food Biotechnology - MICR3215 Flashcards

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1
Q

What are some flaws of current food technology globally?

A

Current food technology globally has flaws such as heavy reliance on fossil fuels and synthetic pesticides and chemical
fertilizers in industrialized countries, leading to environmental concerns.

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2
Q

Despite attempts to increase food production efficiency, what issues still persist in the developing world?

A

hunger and malnutrition

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3
Q

What are some challenges faced by food production worldwide?

A
  1. Arable land becoming scarce,
  2. erosion and salinization stressing crops,
  3. the continuous growth of the human population
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4
Q

Is foodborne illness incidence increasing or decreasing globally?

A

Foodborne illness incidence is increasing in many parts of the world.

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5
Q

How can biotechnology potentially improve food production systems?

A

Biotechnology has the potential to improve food production systems by reducing reliance on chemical pesticides and fertilizers, enhancing food safety, and improving overall efficiency.

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6
Q

What is the purpose of recombinant DNA technology in relation to food?

A

Recombinant DNA technology can be used to directly modify an organism’s genetic structure, allowing control over insect pests, improving nutritional qualities, and enhancing processing characteristics of plant foods.

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7
Q

Apart from modifying organisms, what other role does biotechnology play in food safety?

A

Biotechnology can develop enhanced diagnostic systems to detect pathogens and toxins in food, thereby improving food safety.

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8
Q

How is microbial biotechnology applied in the food industry?

A

Microbial biotechnology is increasingly important in the food industry for obtaining valuable enzymes, amino acids, and polysaccharides from bacteria and fungi.

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9
Q

What are some global food problems?

A
  1. overreliance on fossil fuels,
  2. synthetic pesticides, chemical fertilizers,
  3. hunger and malnutrition,
  4. depletion of arable land,
  5. increase in salinization and erosion,
  6. increasing food production,
  7. an increase in foodborne illness.
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10
Q

How does biotechnology help address these issues?

A
  • the development of transgenic plants which reduce reliance on chemical pesticides
  • the use of diagnostic systems to detect pathogens.
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11
Q

What are some benefits of genetically modified (GM) crops?

A

GM crops have several benefits, including:
- increased crop production,
- reduced carbon dioxide emissions,
- the alleviation of poverty for farmers and their families,
- increased productivity of plants like soybean, canola, corn, and cotton,
- resistance to pests and diseases,
- the production of more nutritious foods like golden rice.

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12
Q

Why is food biotechnology controversial?

A
  1. consumer hostility towards transgenic crops in Europe, Britain, and Japan,
  2. concerns about potential risks to human health and the environment,
  3. the perception that it primarily benefits companies rather than the general public,
  4. public concern stemming from incidents like the contamination of “Starlink” corn in 1999,
  5. criticism of the industry’s use of Golden Rice as a PR tool,
  6. consumer misunderstanding of biotechnology,
  7. the lack of awareness about less controversial aspects such as microbe-derived food additives and DNA-based diagnostic techniques.
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13
Q

What are some examples of food biotechnology that are free from controversy?

A
  • the use of recombinant chymosin in cheese making,
  • xanthan gum in salad dressing,
  • production of ethanol by organisms,
  • diagnostic biotechnology,
  • insulin production.
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14
Q

Why is food security important for every nation?

A

Food security is important for every nation because it is a crucial aspect of economic and political security.

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15
Q

What is the difference in food security between developed and developing countries?

A

Developed nations generally have abundant and high-quality food, while developing countries often face low food security due to a lack of resources and overall food unavailability.

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16
Q

How can biotechnology contribute to improving food security?

A

Biotechnology can improve food security through initiatives such as the development of transgenic plants and better diagnostic systems for detecting and addressing food-related issues.

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17
Q

In how many countries are GM crops grown?

A

GM crops are grown in more than 30 countries around the world.

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18
Q

What is the trend in the global biotech crop area?

A

The global biotech crop area has been increasing for 25 consecutive years, reaching 191.7 million hectares in 2020.

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19
Q

Which countries are the top five in planting GM crops?

A

The top five countries planting GM crops are the United States, Brazil, Argentina, Canada, and India.

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20
Q

What are some commonly grown GM crops?

A

-soybeans,
- corn,
- cotton,
- canola,
- alfalfa.

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21
Q

What are some benefits and applications of GM crops?

A

GM crops have been developed to resist pests, tolerate herbicides, improve nutrition, and provide other benefits in agriculture.

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22
Q

How do consumer attitudes toward GM crops differ?

A

Consumer attitudes toward GM crops vary widely, with some expressing concerns about potential health and environmental impacts, while others view them as a promising tool for sustainable agriculture.

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23
Q

What is the role of lactic acid bacteria in cheese manufacture?

A

Lactic acid bacteria in the starter culture ferment carbohydrates to produce lactic acid.

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24
Q

What happens in the absence of chymosin during cheese production?

A

In the absence of chymosin, decreased pH causes coagulation of casein, resulting in a soft cheese (e.g., cottage cheese).

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25
Q

Why is chymosin required for the production of hard cheese?

A

Chymosin is required for hard cheese production because it increases coagulation by cleaving the casein at amino acids that are attached to carbohydrates.

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26
Q

What was the traditional source of chymosin?

A

The traditional source of chymosin was rennet, which was taken from the stomach of slaughtered cows.

27
Q

Why was the supply and purity of chymosin unreliable when sourced from slaughtered cows?

A

The supply and purity of chymosin from slaughtered cows were unreliable, leading to the need for alternative production methods, because it was dependent on the availability of cows and the enzyme extraction process.

28
Q

How is chymosin produced commercially?

A

Chymosin is produced commercially by cloning the chymosin gene into a microorganism, such as yeast, that can grow on a large scale at low cost.

29
Q

What is the initial form of chymosin produced from its mRNA?

A

The initial form of chymosin produced from its mRNA is the inactive form called preprochymosin, which includes a signal protein portion (“pre”).

30
Q

What happens to preprochymosin to form active chymosin?

A

Preprochymosin is secreted, and the signal portion is removed to form prochymosin. Autolysis then results in the production of active chymosin, which is an active protease.

31
Q

Why was the cloning of the chymosin gene into E. coli problematic?

A

Cloning the chymosin gene into E. coli resulted in clumps of inclusion bodies, making it difficult to obtain the desired chymosin. Purifying and renaturing the inclusions from E. coli was expensive.

32
Q

What was the next organism used for cloning the chymosin gene?

A

The chymosin gene was cloned into yeast, specifically Kluyveromyces lactis, as an alternative to E. coli.

33
Q

What is lactase used for?

A

Lactase is used for the production of lactose-free dairy products by breaking down lactose into glucose and galactose.

34
Q

What was the outcome of cloning the chymosin gene into Kluyveromyces lactis?

A

Cloning the chymosin gene into Kluyveromyces lactis was successful, allowing for the production of chymosin.

35
Q

Is recombinant chymosin considered safe for use in cheese making?

A

The FDA considers recombinant chymosin (rchymosin) to be similar to natural chymosin, so toxicity assessment was not required. Therefore, rchymosin is used in cheese making in many countries, including the US, Canada, and the EU.

36
Q

What was the trade name given to the recombinant bovine growth hormone (rBGH) approved by the FDA?

A

The trade name given to rBGH approved by the FDA was Prosilac®.

37
Q

What was the purpose of cloning bovine growth hormone (BGH) into E. coli?

A

The purpose of cloning BGH into E. coli was to produce large amounts of the recombinant protein (rBGH) for dairy farmers.

38
Q

What was the estimated increase in national milk production as a result of rBGH?

A

The USDA estimated about a 2-5% increase in national milk production due to the use of rBGH.

39
Q

What were some of the concerns raised by opponents regarding the use of rBGH?

A

Opponents raised concerns about the safety of rBGH on human and cattle health, as well as the potential long-term effects that may not be detected in short-term toxicology studies.

40
Q

What did studies show about the presence of insulin-like growth factor (IGF-1) in milk of cows treated with rBGH?

A

Studies showed an increase in the levels of IGF-1 in the milk of cows treated with rBGH.

41
Q

What is the potential role of IGF-1 in relation to tumor formation?

A

IGF-1 can increase the rate of cell proliferation and may have a potential role in tumor formation.

42
Q

Did the FDA approve the sale of rBGH-treated milk?

A

Yes, the FDA approved the sale of rBGH-treated milk, stating that the scientific evidence indicated that the amount of IGF-1 in milk was safe

43
Q

Are there any countries that still do not allow the sale of rBGH-treated milk?

A

Yes, some countries, including the EU, do not allow the sale of rBGH-treated milk.

44
Q

What are some of the concerns raised by consumers regarding the use of recombinant proteins in food?

A

Consumers may be offended by the concept of “supercharging” animals and the perceived “unnatural” origin of recombinant proteins. They may also question the direct benefit to the consumer, which can lead to resentment.

45
Q

Why is fruit ripening essential for food production?

A

Unripened fruit is usually inedible.

46
Q

What are the changes associated with fruit ripening?

A

texture, structure, and flavor.

47
Q

What are the two choices to overcome transport stress in ripened fruit?

A

The two choices are growing hardy fruit cultivars or transporting fruit in an unripened state.

48
Q

What triggers a burst of respiratory activity during ripening in climacteric fruits?

A

Increased production of ethylene triggers the burst of respiratory activity.

49
Q

What is the main cause of softening in fruits?

A

Softening is primarily caused by the degradation of pectin by polygalacturonase (PG).

50
Q

How can transgenic fruits with delayed ripening be created?

A

Transgenic fruits with delayed ripening can be made by reducing the amount of PG or ethylene produced using antisense technology.

51
Q

What is the method used to deliver the recombinant construct into tomatoes?

A

Agrobacterium is used as the delivery system to deliver the recombinant construct into the tomatoes.

52
Q

What is the purpose of developing Flavr Savr tomatoes?

A

Flavr Savr tomatoes have lower levels of PG, resulting in a decreased rate of softening after harvest.

53
Q

How can the ripening of tomatoes be inhibited?

A

Inhibition of ethylene production is an alternative approach to inhibiting softening in tomatoes.

54
Q

What is the main application of transgenic tomatoes with antisense ACC oxidase genes?

A

The main application is to reduce fruit loss during transport.

55
Q

How is vitamin A produced in humans and vertebrate animals?

A

Vitamin A is produced from carotenoids, with β-carotene being the most common precursor.

56
Q

What is the purpose of developing Golden Rice?

A

Golden Rice was developed to address vitamin A deficiency by increasing the levels of β-carotene in rice.

57
Q

What was challenging in developing Golden Rice?

A

Three enzymes are required to convert GGPP to β-carotene, and none of these enzymes is active in the rice endosperm.

58
Q

What are the enzymes used in the production of Golden Rice and where do they originate from?

A
  1. Phytoene synthase- psy (daffodil)
  2. Lycopene cyclase - lcy (daffodil)
  3. Beta carotene desaturase - crt ( Erwinia uredovora)
59
Q

How were the genes for the required enzymes inserted into rice?

A

Genes for two enzymes (psy and crt) were inserted into one plasmid vector (CR1) , and the other gene (lcy) was inserted into a separate plasmid (CR2).

60
Q

How were the transformed plant cells selected during Golden Rice development?

A

Transformed plant cells were selected using hygromycin, a selectable marker gene.

61
Q

What is the average β-carotene content in a typical line of Golden Rice?

A

In a typical line, the average β-carotene content was 1.6 µg/g of endosperm tissue.

62
Q

What are the visual symptoms of papaya ringspot virus infection?

A

The visual symptoms include a yellow mosaic pattern on the leaves, shoe string appearance in fruits and leaves, and rings on the surface of the fruit.

63
Q

How was pathogen-derived resistance achieved in papaya?

A

he coat protein gene from papaya ringspot virus was isolated and introduced into the plants, resulting in resistance to infection

64
Q

What is the method used to clone the Papaya ringspot virus coat protein gene?

A

The gene is cloned into a plasmid under the control of the 35S promoter, a constitutive promoter.