fluorescence Flashcards

1
Q

applications

A
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2
Q

quantum yield

A
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3
Q

morse curve

A
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4
Q

spec

A

light scatters=> better data at 90 degrees

only fraction of signal received

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5
Q

solvent effect

A
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6
Q

sample prep

A
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7
Q

blanks and control

A
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8
Q

measurement

A
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9
Q

troubleshooting

A
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10
Q

excitation spectra

A
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11
Q

measuring exc spec

A
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12
Q

interpreting spec

A
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13
Q

inner filter effect

A
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14
Q

self-Q

A
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15
Q

dynamic vs static Q

A
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16
Q

calcein leakage

A
17
Q

laurdan

A

solvent sensitive dye

as M melts more hydrophilic and fluid

18
Q

laurdan 2

A
19
Q

Ligand binding app

A
20
Q

Spec titration

A
21
Q

tight binding

A
22
Q

N determination

A
23
Q

alternative way to get N

A
24
Q

moderate binding

A
25
Q

N for moderate or weak binding

A
26
Q

2nd exp

A
27
Q

non-linear fitting

A
28
Q

titration with indicator

A
29
Q
A