Flow panels intro

Question 1

hematogones are - and pos for

Answer 1

lymphoid precursors

CD34

Question 2

First gate

Answer 2

CD45 vs SSC

Question 3

CD45

Answer 3

leukocyte common antigen

Question 4

CD14

Answer 4

mature monocyte marker

Question 5

CD3 and NK cells

Answer 5

NK cells can express CD3 in the cytoplasm

Question 6

CD4 and CD8 ratio is always measured in the

Answer 6

p blood

Question 7

NK cell markers

Answer 7

CD16 and 56

Question 8

Pan T cell markers other than CD3

Answer 8

CD2, CD5 and CD7

Question 9

How to detect clonality in B cells

Answer 9

k or l

Question 10

CD25

Answer 10

activated T cells

adult T cell lymphoma

Question 11

HLA DR

Answer 11

all B cells

activated T cells (?), monocytes

Question 12

CD30

Answer 12

anaplastic large T cell lymphoma

also activation marker

Question 13

freq of TCR a/b and g/d

Answer 13

95%

lt 5% g/d

Question 14

g/d TCR seen in

Answer 14

aggressive lymphoma such as hepato splenic T cell lymphoma

Question 15

nu TdT

Answer 15

marker of immaturity

Question 16

cy MPO

Answer 16

lineage marker

Question 17

CD19 vs CD20

Answer 17

both are lineage associated I think

all B cell lymphomas express this

Question 18

CD20 kinetics

Answer 18

on mature B cells and loose when become plasma cells

Question 19

CD10

Answer 19

very early on B cells: when it is lost, called naive circulating B cells

Question 20

which cells express CD10 other than B cells

Answer 20

segmented neutrophils

if they don’t have it, abn myeloid hyperplasia

Question 21

CD34 and blast cells, eg. of leukemia

Answer 21

blast cells express CD34

ALL B type

Question 22

in panel 10, CD45 is replaced by

Answer 22

CD20 for gating.

then look at CD5 pos

Question 23

CD43

Answer 23

pan hematopoetic marker (even nRBC and plasma cells but not B cells express)
Certain CLL and SLL

Question 24

CD103

Answer 24

intra-epithelial lymphocyte

hairy cell leukemia

Question 25

CD23 and CD5

Answer 25

both pos for CLL and SLL

Question 26

CD38 is used to differentiate between

Answer 26

pre and post germinal center cells (pre- and post mutated)

Question 27

FMC17

Answer 27

linked to CD20 complex on surface of B cells

Question 28

CD11c

Answer 28

myelo monocytic associated marker

splenic type B cell lymphoma

Question 29

isotype of heavy chain is not used to assess

Answer 29

clonality

Question 30

CD117

Answer 30

blasts are pos for CD117, CD34 and CD33

Question 31

CD64

Answer 31

lineage associated monocytes

Question 32

CD1a

Answer 32

langerhans histiocytosis

? T -ALL

Question 33

CD38 vs CD138 top and bottom

Answer 33

top plasma cells
bottom neuroendocrine (check if CD56 pos)

Question 34

plasma cell repertoire

Answer 34

CD45 +/dim CD56 -/dim 
CD19+ CD20-
CD27+ CD28- 
CD81+ CD117-
k twice as l