Flash cards

1
Q

Ael

A

Antigen only detectable by adsorption/elution

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2
Q

Ay

A

Does not agglutinate serologically. Do have substances in secretions.

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3
Q

Am

A

Does not react strongly serologically

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4
Q

Ax

A

Does not agglutinate with anti-a. Weak agglutination with Anti-A,B

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5
Q

A-end

A

<= 10% of RBCs show mixed field agglutination

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6
Q

A terminal sugar

A

N-Acetyl galactosamine

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7
Q

B terminal sugar

A

Galactose

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8
Q

Most to least common ABO types

A

O (50%)>A(35%)>B(15%)>AB (5%)

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9
Q

A3

A

Mixed field agglutination

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10
Q

Dolichos Biflorus

A

A1 lectin

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11
Q

Bandeiraea simplicifolia

A

B lectin

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12
Q

Ulex Europaeus

A

H lectin

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13
Q

Galactosamine

A

Acquired B sugar

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14
Q

Anti-A: 4+
Anti-B: 0
Anti-A,B: 4+
A1 Cell: 0
B Cell: 4+

A

Type A

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15
Q

Anti-A: 0
Anti-B: 4+
Anti-A,B: 4+
A1 Cell: 4+
B Cell: 0

A

Type B

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16
Q

Anti-A: 0
Anti-B: 0
Anti-A,B: 0
A1 Cell: 4+
B Cell: 4+

A

Type O

Can be Bombay too

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17
Q

Anti-A: 4+
Anti-B: 4+
Anti-A,B: 4+
A1 Cell: 0
B Cell: 0

A

Type AB

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18
Q

When to use/Not use
A1 Lectin

A

Use: Weak Expression of A
Not use: No Expression of A

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19
Q

When to use/not use
A2 Cells

A

Use: Patient has extra reaction with A1 Cells
Not use: no reaction with A1 cells

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20
Q

When to use/not use
Anti-A,B

A

Use: Weak reaction with Anti-a/Anti-B
Not use: Normal reactivity with Anti-A/Anti-B

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21
Q

When to use/not use
O cells

A

Use: Extra antibody reactivity with A/B antibody
Not Use: Weaker antibody activity

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22
Q

When to use/not use
Patient cells

A

Use: Extra antibody activity related to all other cells
Not use: weaker antibody reaction

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23
Q

Plasma Donation

A

Mirrors blood donation

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24
Q

FUT3 gene

A

Codes for Lewis Transferase

25
T/F: Lewis Antigens are an antithetical pair
False
26
FUT gene location
Chromosome 19
27
FUT 3 Only
Lea
28
FUT2 + FUT3
Leb
29
T/F: Rh factor is always associated w/ HDN
True
30
Most immunogenic antigen
Rh (80% Antibody formation)
31
RhNegative vs. RhNULL
Negative: No D antigen expressed Null: No Rh family expression, including C,E, RaHG
32
Weak DU
Low Antigen expression inside cell Do NOT make Anti-D
33
Partial D
Mutation in the D antigen Outside Cell CAN make Anti-D (to the mutated section)
34
Anti-D Ig Type
IgG
35
Rh D Chromosome
Chromosome 1
36
Units of blood needed calculation
100 - frequency (each if more than 1 frequency) = Compatibility (compatibility #1)(Compatibility #2).... compatible/100 = 1/x. Solve for X
37
RhNULL Serology
Lack Rh Antigens Lack LW & FYS Reduced expression of SsU
38
D Frequency (calculation)
85% (Lower in Whites)
39
C Frequency (calculation)
70% (Lower in Black population)
40
c Frequency (calculation)
80 (Lower in Asian population)
41
E Frequency (calculation)
30%
42
e Frequency (calculation)
98%
43
R0/Dce Demographic
Black population
44
R1/DCe Demographic
Asian (70%)/White (42%)
45
R2/DcE Demographic
Asian (21%)/White (14%)
46
r/dce Demographic
White (37%)/Black (26%)
47
Anti-F
ce on same haplotype
48
Anti-G
D or C on haplotype
49
Coomb's Test Positive
Positive in IS or 37C or AHG
50
Coomb's Test Negative
Positive only in check cell step
51
Coomb's Test Invalid
Negative in Check cell step
52
Enhancement effects
LISS - Zeta potential Polybrene - Forms a bridge Albumin - -Beach Ball Polyethylene Glycol - Reduces H2O
53
What doesn't Gel require
Wash step
54
Reverse Cells
Mixture of Donors of A or B type
55
Screen cells
Single donor of O type and known antigens
56
B3
Mixed field with anti-B and anti-A,B
57
Bx
Weak rxn w/ Anti-B and Anti-A,B Weakly reactive anti-B
58
Bm
More frequent in Japan Normal amount of B substance in secretions No anti-B detected
59
Bel
Must be detected by adsorption and elution