Flash cards

1
Q

Ael

A

Antigen only detectable by adsorption/elution

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2
Q

Ay

A

Does not agglutinate serologically. Do have substances in secretions.

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3
Q

Am

A

Does not react strongly serologically

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4
Q

Ax

A

Does not agglutinate with anti-a. Weak agglutination with Anti-A,B

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5
Q

A-end

A

<= 10% of RBCs show mixed field agglutination

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6
Q

A terminal sugar

A

N-Acetyl galactosamine

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7
Q

B terminal sugar

A

Galactose

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8
Q

Most to least common ABO types

A

O (50%)>A(35%)>B(15%)>AB (5%)

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9
Q

A3

A

Mixed field agglutination

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10
Q

Dolichos Biflorus

A

A1 lectin

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11
Q

Bandeiraea simplicifolia

A

B lectin

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12
Q

Ulex Europaeus

A

H lectin

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13
Q

Galactosamine

A

Acquired B sugar

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14
Q

Anti-A: 4+
Anti-B: 0
Anti-A,B: 4+
A1 Cell: 0
B Cell: 4+

A

Type A

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15
Q

Anti-A: 0
Anti-B: 4+
Anti-A,B: 4+
A1 Cell: 4+
B Cell: 0

A

Type B

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16
Q

Anti-A: 0
Anti-B: 0
Anti-A,B: 0
A1 Cell: 4+
B Cell: 4+

A

Type O

Can be Bombay too

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17
Q

Anti-A: 4+
Anti-B: 4+
Anti-A,B: 4+
A1 Cell: 0
B Cell: 0

A

Type AB

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18
Q

When to use/Not use
A1 Lectin

A

Use: Weak Expression of A
Not use: No Expression of A

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19
Q

When to use/not use
A2 Cells

A

Use: Patient has extra reaction with A1 Cells
Not use: no reaction with A1 cells

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20
Q

When to use/not use
Anti-A,B

A

Use: Weak reaction with Anti-a/Anti-B
Not use: Normal reactivity with Anti-A/Anti-B

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21
Q

When to use/not use
O cells

A

Use: Extra antibody reactivity with A/B antibody
Not Use: Weaker antibody activity

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22
Q

When to use/not use
Patient cells

A

Use: Extra antibody activity related to all other cells
Not use: weaker antibody reaction

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23
Q

Plasma Donation

A

Mirrors blood donation

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24
Q

FUT3 gene

A

Codes for Lewis Transferase

25
Q

T/F: Lewis Antigens are an antithetical pair

A

False

26
Q

FUT gene location

A

Chromosome 19

27
Q

FUT 3 Only

A

Lea

28
Q

FUT2 + FUT3

A

Leb

29
Q

T/F: Rh factor is always associated w/ HDN

A

True

30
Q

Most immunogenic antigen

A

Rh (80% Antibody formation)

31
Q

RhNegative vs. RhNULL

A

Negative: No D antigen expressed
Null: No Rh family expression, including C,E, RaHG

32
Q

Weak DU

A

Low Antigen expression
inside cell
Do NOT make Anti-D

33
Q

Partial D

A

Mutation in the D antigen
Outside Cell
CAN make Anti-D (to the mutated section)

34
Q

Anti-D Ig Type

A

IgG

35
Q

Rh D Chromosome

A

Chromosome 1

36
Q

Units of blood needed calculation

A

100 - frequency (each if more than 1 frequency) = Compatibility

(compatibility #1)(Compatibility #2)….

compatible/100 = 1/x. Solve for X

37
Q

RhNULL Serology

A

Lack Rh Antigens

Lack LW & FYS

Reduced expression of SsU

38
Q

D Frequency (calculation)

A

85% (Lower in Whites)

39
Q

C Frequency (calculation)

A

70% (Lower in Black population)

40
Q

c Frequency (calculation)

A

80 (Lower in Asian population)

41
Q

E Frequency (calculation)

A

30%

42
Q

e Frequency (calculation)

A

98%

43
Q

R0/Dce Demographic

A

Black population

44
Q

R1/DCe Demographic

A

Asian (70%)/White (42%)

45
Q

R2/DcE Demographic

A

Asian (21%)/White (14%)

46
Q

r/dce Demographic

A

White (37%)/Black (26%)

47
Q

Anti-F

A

ce on same haplotype

48
Q

Anti-G

A

D or C on haplotype

49
Q

Coomb’s Test Positive

A

Positive in IS or 37C or AHG

50
Q

Coomb’s Test Negative

A

Positive only in check cell step

51
Q

Coomb’s Test Invalid

A

Negative in Check cell step

52
Q

Enhancement effects

A

LISS - Zeta potential
Polybrene - Forms a bridge
Albumin - -Beach Ball
Polyethylene Glycol - Reduces H2O

53
Q

What doesn’t Gel require

A

Wash step

54
Q

Reverse Cells

A

Mixture of Donors of A or B type

55
Q

Screen cells

A

Single donor of O type and known antigens

56
Q

B3

A

Mixed field with anti-B and anti-A,B

57
Q

Bx

A

Weak rxn w/ Anti-B and Anti-A,B
Weakly reactive anti-B

58
Q

Bm

A

More frequent in Japan
Normal amount of B substance in secretions
No anti-B detected

59
Q

Bel

A

Must be detected by adsorption and elution