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Histopathology (Lecture) Pathology & Fixative > Fixative > Flashcards

Fixative Flashcards

1
Q

Recommended for MAILING SPX and for COLORED TISSUE PHOTOGRAPHY

A

FORMALDEHYDE/FORMALIN

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2
Q

Removal of Formalin Pigments:
70% ethanol & 28% ammonia water

A

Kardasewitsch method

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3
Q

Removal of Formalin Pigments:
H2O2 + 28% ammonia water + Acetone

A

Lillie’s method

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4
Q

Removal of Formalin Pigments:
Saturated alcoholic picric acid

A

Picric acid method

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5
Q

Remedy for precipitation of white paraformaldehyde

A

10% Methanol

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6
Q

Distilled water w/ Sodium chloride (Histochemical fixative)

A

10% formol saline

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7
Q

Used for general post mortem tissues and CNS tissues

A

10% formol saline

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8
Q

10% formalin + phosphate buffer

A

10% neutral buffered formalin

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9
Q

Recommended for tissues w/ iron pigments and elastic fibers

A

10% neutral buffered formalin

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10
Q

Also known as Formol sublimate. Contains formaldehyde + mercuric chloride

A

Formal corrosive

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11
Q

Recommended for lipids, neutral fats, and phospholipids

A

Formal corrosive

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12
Q

Also known as Gender’s solution. 95% ETOH + Picric acid + Glacial acetic acid

A

Alcoholic formalin

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13
Q

For enzyme histochemistry and electron microscopy

A

Glutaraldehyde

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13
Q

Recommended for Sputum specimens and microincineration techniques

A

Alcoholic formalin

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14
Q

Glutaraldehyde solution % for small fragments and % for larger tissues less than 4mm thick

A

2.5% for small fragments; 4% for larger tissues

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15
Q

Glutaraldehyde solutions for electron microscopy

A

Karnovsky’s paraformaldehyde-glutaraldehyde solution and Acrolein

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16
Q

Supplied as 40% aqueous solution. Smallest aldehyde fixative

A

Glyoxal

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17
Q

Glyoxal fixative duration for Surgical specimens and Small biopsy specimens

A

4-6 hours; 45 mins

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18
Q

Most common metallic fixative

A

Mercuric chloride

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19
Q

Mercuric chloride + Glacial acetic acid.

Recommended for liver, spleen, connective tissue fibers, and nuclei.

A

Zenker’s fluid

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20
Q

Recommended for pituitary gland, bone marrow, blood containing organs

A

Zenker’s formol/ Helly’s fluid

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20
Q

Potassium dichromate + 40% formaldehyde

Also known as Helly’s fluid

A

Zenker’s formol/ Helly’s fluid

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21
Q

TCA + Glacial acetic acid + formalin

Recommended for tumor skin biopsies

A

Heidenhain’s susa

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22
Q

Anhydrous sodium acetate

For preserving bone marrow

22
Q

Chromate fixative: preserves carbohydrates

A

(1-2%) Chromic acid

22
Q

Mercuric acid fixative may form black mercury deposits, remedy?

A

wash with Alcoholic iodine

23
Q

For early degenerative processes and tissue necrosis.

For Rickettsia and other bacteria

A

Orth’s fluid

23
Q

Chromate fixative: preserves lipids and mitochondria

A

3% Potassium dichromate

23
Q

Recommended for chromatin, mitochondia, mitotic figires, golgi bodies, rbc containing colloid tissues

A

Regaud’s / Moller’s fluid

23
Q

Used for acid mucopolysaccharide and tissue mucin

A

Lead fixative

24
Q

Acts as a Fixative, Stain, Decalcifying agent. Excellent in Glycogen demonstration

A

Picric acid fixatives

25
Q

This fixative stains the tissue YELLOW as a major drawback

A

Picric acid fixatives

26
Q

Recommended for embryos, pituitary biopsy, endometrial biopsy, endometrial curettings.

A

Bouin’s solution

27
Q

Excellent for Glycogen

A

Brasil’s alcoholic picformol

28
Q

For GIT biopsies and endocrine tissues

A

Hollande’s solution

29
Q

A compound fixative, recommended for nucleoproteins

A

Glacial acetic acid

30
Q

Glacial acetic acid solidifies at ___ and contraindicated for cytoplasmic fixation

31
Q

Acts as a Fixative and Decalcifying agent. A compound fixative.

A

Trichloroacetic acid (TCA)

31
Q

Fixative is used at ice cold temperatures -5 to 4C

32
Q

Recommended for Enzymes and Brain tissues to diagnose Rabies

33
Q

Can Fix and Dehydrate at the same time

A

Acetone and Alcohol

34
Q

Rapidly denatures and precipitates proteins

35
Q

Ideal for small tissue fragments.

Causes Glycogen polarization

A

Alcohol fixatives

36
Q

Alcohol fixative: for wet and dry smears, blood smears, and bone marrow tissues

A

100% Methyl alcohol

37
Q

Recommended for touch preparations

A

95% Isopropyl

38
Q

Recommended for blood films, tissue films, and smears

A

70-100% Ethyl alcohol

39
Q

Alcohol + Glacial acetic acid + chloroform

A

Carnoy’s

40
Q

Most rapid fixative.

For chromosomes and lymph glands.

w/ acetone to fix brain tissues to diagnose rabies

A

Carnoy’s

41
Q

Classified as both nuclear (nuclear proteins) and histochemical (mucopolysaccharide) fixative

A

Newcomer’s

42
Q

Recommended for CT mucins and umbilical cord

A

Rossman’s solution

43
Q

This fixative is Expensive, slow-acting, used in EM.

A

Osmium tetroxide

44
Q

Processes Neurological tissues.

For myelin and peripheral nerves

A

Osmium tetroxide

45
Q

Most common chrome osmium acetic acid fixative.

For nuclear structures

A

Flemming’s

46
Q

Osmium tetroxide fixative: for cytoplasmic structures

A

Flemmings without acetice acid

47
Q

3 Fixatives for Electron Microscopy

A

Glutaraldehyde, Osmium tetroxide, Paraformaldehyde

48
Q

2 Fixatives for Enzyme Histochemistry

A

4% Formaldehyde, formol saline

49
Q

Fixative for Electron histochemistry and Electron immunocytochemistry

A

Karnovsky’s paraformaldehyde-glutaraldehyde

49
Q

Human brain must undergo intravascular perfusion and washed with?

A

Ringer’s Lactate

49
Q

Hard tissues (cervix, uterine, fibroid) must undergo what method?

A

Lendrum’s method (immersing the spx in 4% aqueous phenol for 1-3 days)

Histopathology (Lecture) Pathology & Fixative (2 decks)

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