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Histopathology > FIXATION > Flashcards

FIXATION Flashcards

1
Q

Action of fixative where the fixative becomes part of the tissue

A

Additive

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2
Q

Action of additive fixative

A

Formation of crosslinks or complexes

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3
Q

Fixative not incorporated into the tissue

A

Non-additive

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4
Q

Action of non additive tissue fixative

A

Stabilizies tissue by removing water bound water

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5
Q

pH in fixation

A
  1. 0-8.0
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6
Q

pH in fixation

A
  1. 0-8.0
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7
Q

Temperature in regular fixation

A

Room temp (22-25)

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8
Q

Temperature of autotech fixation

A

40 C

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9
Q

Temp of EM and histochem fixation

A

0-4 C

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10
Q

Temperature in rapid fixation

A

60 C

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11
Q

Fixation temp in specimens with TB

A

100 c

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12
Q

Thickness of tissues in EM

A

1-2 mm

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13
Q

Thickness of tissue in LM

A

2cm

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14
Q

Tissue thickness should not be bigger than ____

A

4mm or 0.4cm

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15
Q

Brain tissue fixation prep suspended in ______

A

Circle of willis = 10 % buffered formalin 2-3weeks

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16
Q

Solution concentration in fixation

A

Hypertonic ( 400-450osm)

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17
Q

Ideal concentrating reagent in immunoelectrochemistry

A

0.25 glutaraldehyde

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18
Q

Time of fixation to prevent decomposition

A

Less than 1 hr

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19
Q

Rate of penetration of formalin

A

1mm/hr

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20
Q

Volume in fixation

A

20 times of tissue

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21
Q

Volume of fixative for expensive fixatives

A

5-10x of tissue

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22
Q

Volume for museum fixation

A

> 50x

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23
Q

Mortuary refrigerator temp

A

4c

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24
Q

Organ grossing is done after fixation

A

Brain

25
Q

Organ Injected and cut after fixatiom

A

Eyes

26
Q

Fixative that permit the general microscopic study of tissue structures without altering the structural pattern

A

Microanatomical

27
Q

Preserves specific part or element of cell

A

Cytological

28
Q

Needs glacial acetic acid

A

Nuclear

29
Q

Effect of glacial acetic acid to cytoplasm

A

Swelling

30
Q

Produced from oxidation of methanol

A

Formaldehyde

31
Q

Pure stock of formalin sol

A

37-40%

32
Q

Commonly used formalin sol

A

10%

33
Q

Fixation time of formalin

A

12-24 hrs

34
Q

Added to 10% formaline as buffer

A

PO4

35
Q

Result of prolonged storaged of 10 formaline

A

Paraformaldehyde (white precipitate)

36
Q

Black or brown precipitates im formaline solution in blood containing tissues

A

Acid formaldehyde hematein

37
Q

Ideal for silver impregnation techniques

A

10% formaline

38
Q

Best fixative for iron containing pigments

A

10% neutral buffered formaline

39
Q

Recommended for routine histopath

A

10% neutral buffered formaline

40
Q

Excellent for stains like reticulin mtds

A

Formol corrosive (formal sublimate)

41
Q

Good for glycogen and microinceneration techniques

A

Gendre’s

42
Q

Fixes sputum

A

Gendre’s

43
Q

2 formaljne linked by 3 carbon chains

A

Glutaraldehyde

44
Q

Recommended for enzyme histochem and EM

A

Glutaraldehyde

45
Q

Polymer of formalin in white powder form

A

Paraformaldehyde

46
Q

Used for ultra thin plastic embedding

A

Paraformaldehyde

47
Q

Most common metallic fixative

A

Mercuric

48
Q

Mercuric fixative that does not produce black granular deposits

A

Heidenhain susa

49
Q

Removal for black deposits in mercuric chloride fixation

A

Dezenkerization

50
Q

Routine fixative of choice for preservatiln of cell detail in tissue photography

A

Mercuric chloride

51
Q

Mercuric chloride that contains glacial acetic acid

A

Zenker’s

52
Q

Mercuric chloride fixative Recommended for trichrome stain

A

Zenker’s

53
Q

Mercuric stain that produces brown pigments

A

Zenker formol

54
Q

Excellent microanatomical fixative of pituitary gland, BM and blood containing organs

A

Zenker formol

55
Q

Other name of zenker formol

A

Helly’s

56
Q

Mercuric stain recommended for skin tumor

A

Heidenhain susa

57
Q

Mercuric fixatice with anhydrous Na acetate

A

B5 fixative

58
Q

Mercuric stain for bone marrow

A

B5

Histopathology (2 decks)

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