Fixation Flashcards
Mechanism in fixation wherein the fixing agent is not incorporated into the tissue
Non-additive fixation
Mechanism in fixation wherein the fixing agent is taken in and becomes part of the tissue
Additive fixation
Inadequate or poor fixation will result in?
Poorly processed tissue
Disadvantage of liquid nitrogen
Soft tissue is liable to crack
Fixation helps preserve ____, ____, ____, and _____ of tissue
Shape, structure, intercellular relationship, and chemical constituents
Generally used in histochemistry and during operative procedures
Liquid nitrogen
Leaving the tissue in water will result in?
Cell shrinkage
Amount of fixative to be used for maximum effectiveness of fixation is?
20x the tissue volume
The following are additive fixatives except:
A. Formalin
B. Mercury
C. Alcohol
C. Alcohol
Types of fixative according to composition
Simple fixative and compound fixative
Type of fixative which permits the general microscopic study of tissue structures without altering the structural pattern and normal intercellular relationship of tissue in question
Microanatomical fixative
Type of fixative which has been added together to obtain optimal combined effect of their individual actions
Compound fixative
Type of cytological fixative which preserves the nuclear structures and usually contains glacial acetic acid as primary component
Nuclear fixative
The following are nuclear fixative except for two A. Flemming’s fluid B. Orth’s fluid C. Heidenhain’s susa D. Regaud’s fluid E. Bouin’s fluid
B and D. Orth’s and Regaud’s fluids are cytoplasmic fixatives
Leaving the tissue in air for prolonged period of time will cause?
Drying out and will further result in distortion of its morphologic appearance.