Brainscape's Knowledge GenomeTM

Browse over 1 million classes created by top students, professors, publishers, and experts.


See full index

Histopath > fixation > Flashcards

fixation Flashcards

1
Q

10% Formal saline is also recommended for the preservation of lipids and phospholipids (true or false)

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

Fixation time for 10% formol saline

A

12-24 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

Recommend for general post mortem tissue and CNS

A

10% formol saline

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Fixative tissue containing iron pigments and elastic fibers

A

10% Neutral buffered formalin

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

Alternatives to mercuric chloride. They are said to give improved results with immunohistochemistry

A

Zinc Formalin (Unbuffered)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

Formol corrosive Fixation time?

A

3-24 hrs

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

It fix lipids, neutral fats, and phospholipids. There is no need for “washing out”

A

Formol Corrosive (Formol sublimate)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

Polymerized form of formaldehyde, usually obtained as fine white powder. Suitable form paraffin embedding and sectioning, also for immunocytochemical analysis

A

Paraformaldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

Mixture of paraformaldehyde and glutaral-dehyde. It is suitable for use when preparing sample for light microscopy.

A

Karnovsky’s Fixative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

Made up of two formaldehyde residue, linked by three carbon chain. Well suited for EM. NOT good for immunohistocehmical staining

A

Glutaraldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

Fixative for Enzyme Histochemistry

A

4% formaldehyde
Formol Saline

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Fixative for EM

A

Glutaraldehyde
Osmium tetroxide
Paraformaldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Fixative for electron histochemistry and Electron immunocytochemistry

A

Karnovsky’s paraformaldehyde- glutaraldehyde

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Alcohols are protein denaturants and are not used routinely for tissue (true or false)

A

TRUE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Washing out using TAP WATER

A

excess; chromates, hellys, zenker’s, Flemmings, Formalin, osmic acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Washing out using alcoholic iodine

A

for mercuric fixative

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
17
Q

Washing out 50-70% alcohol

A

picric acid

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
18
Q

Excellent for fixing dry and wet smear, blood smears and bone marrow tissue

A

Methyl Alcohol 100%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
19
Q

Fixing TOUCH preparation, blood? tissue film

A

Isopropyl Alcohol 95%

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
20
Q

Ethyl alcohol preserve and fix glycogen (true or false)

A

FALSE (it preserved but does NOT fix glycogen)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
21
Q

For blood, tissue films and smear

A

Ethyl Alcohol (70-100%)

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
22
Q

Fixation time for ethyl alcohol?

A

18-24 hrs

23
Q

Fixation time for carnoy’s

A

1-3 hrs. fast acting fixative

24
Q

Use for urgent biopsy. Fix chromosomes and lymph glands. Use with acetone for brain tissue for diagnosis of rabbies

A

Carnoy’s

25
Q

Carnoy’s contain _____, ______, and _____

A

Absolute alcohol, Chloroform, Glacial acetic acid

26
Q

Used on frozen section and smear. It preserve nucleic acid but extracts lipids

A

Clarke’s solution

27
Q

For sputum and microincineration technique

A

Gendre’s solution

28
Q

_____ combines a denaturing fixative with the additives and cross-linking effects of formalin. It can be used for fixation or post-fixation of large fatty specimen (particularly breast)

A

Alcoholic formalin

29
Q

Classified as nuclear (nuclear protein) and histochemical fixative (mucopolysaccharide).

A

Newcomers fluid

30
Q

Connective tissue mucins and umbilical cords

A

Rossmann’s solution

31
Q

Most common metallic fixative

A

Mercuric chloride

32
Q

Zenker’s Solution is recommended for trichrome staining (true or false)

A

TRUE

33
Q

Recommend for congested specimen (lungs, heart, and blood vessels) and give good results with PTAH and trichrome staining

A

Zenker’s solution

34
Q

contains mercuric chloride and glacial acetic acid. Recommend in fixing liver, spleen, and connective tissue fibers and nuclei

A

Zenker’s fluid

35
Q

Preserve pituitary gland, bone marrow, and other blood containing organs

A

Zenker-Formol (Helly’s)

36
Q

tumor skin biopsy; it is an excellent cytologic fixative

A

Heidenhain’s Susa solution

37
Q

Pale yellow powder which dissolve in water to form a strong oxidizing solution.

A

Osmium tetroxide

38
Q

required volume for osmium tetroxide

A

5 to 10x the volume of the specimen

39
Q

Osmium tetroxide is a good fixative and excellent stain for lipids in membranous structure and vesicle. (True or False)

A

TRUE

40
Q

For ricketessia and other bactera
For early degenerative process and tissue nectrosus

A

Orth fluid

41
Q

To fix myelin, peripheral nerves and neurological tissue

A

Osmium tetroxide

42
Q

Most common chrome-osmium acetic acid fixative used, recommended for nuclear preparation of such sections

A

Flemming’ s solution

43
Q

is made up only of chromic and osmic acid, recommended for cytoplasmic structures particularly the mitochondria

A

Flemming’ s solution without acetic acid

44
Q

used on 1-2% aqueous solution, usually as a constituent of a compound fixative. Preserve CARBOHYDRATES

A

Chromic acid

45
Q

chromate fixatives that preserve lipids and mitochondria (3% aqueous solution)

A

Potassium dichromate

46
Q

Recommend for demonstration of CHROMATIN, MITOCHONDRIA, MITOTIC FIGURES, GOLGI BODIES, RBC & COLLOID-CONTAINING tissues

A

Regaud’ s (Moller’ s) Fluid

47
Q

Recommend for fixation of embryos, pituitary biopsy, and endometrial curetings

A

Bouin’ s solution

48
Q

Bouin’s solution is not suitable for Aniline stains but stable (True or False)

A

FALSE (it is suitable for Aniline stain)

49
Q

recommend for GIT and endocrine tissue

A

Hollande’s solution

50
Q

Hollande’s solution has some decalcifying properties. (True or False)

A

TRUE

51
Q

types of picric acid that is excellent fixative for glycogen

A

Brasil’ s Alcoholic Picroformol fixative

52
Q

Preserve acid mucopolysaccharide and tissue mucin

A

Lead Fixative

53
Q

used for precipitating of proteins and nucleic acids. Also used as a decalcifier and fixative in microscopy

A

Trichloroacetic acid

54
Q

provide stable medium for transport of fresh unfixed tissue, such as renal, skin and oral mucosa biopsies.

A

Michel’ s solution

Histopath (1 decks)

Brainscape helps you reach your goals faster, through stronger study habits.
© 2024 Bold Learning Solutions. Terms and Conditions