FISH Probe Kit

Question 1

Detects ALK gene rearrangements in ………..

Answer 1

FFPE NSCLC tissue

Question 2

Score percent break apart or percent fused in ……….. or more tumor cells

Answer 2

50

Question 3

ALK pos. samples in ….% scoreable nuclei show break apart signal

Answer 3

15

Question 4

Techs must be capable of distinguishing between ……………… color signals

Answer 4

orange, green, and yellow (fused orange/green) signals

Question 5

Testing time

Answer 5

• at least 6 hours fixation

- 14-24hrs for hybridization, semi-automated

Question 6

visualization

Answer 6

fluorescence microscope