FISH AND EGG Flashcards
Bulging or prominent eyes, transparent cornea, black pupil
fresh fish
Slightly sunken cornea, somewhat full pupil
grade 2
Sunken cornea, dull and milky white pupil
grade 3
Much sunken heavy yellow slime, opaque cornea
grade 4
utilization of grade 2
bagoong, drying, smoking
utilization of grade 4
rendering plant, fish meal or fertilizer
utilization of grade 3
condemnation/patis manufacture
utilization of grade 1
quick sale for local market
Slight smell like rotten cabbage turnip or melon-like slight smell of ammonia
grade 3
Bleaching or greenish brown discoloration, heavy
grade 4
muddy, milky slime, “bloom” fades
grade 2
Light slime, no discoloration; scales intact and adheres to skin, in “full bloom”
grade 1
In fish intended for drying, formalin is being used to
get rid of worms and make the scales glow.
Smoked fish and meat product can naturally contain formalin from the wood smoke or from artificial smoking by smearing with wood distillate (“liquid smoke”)
true
preliminary test for detection of formalin
amidol test
he amidol test is not specific for formaldehyde as it also produces reaction with other aldehydes. For this reason, positive reaction should be further confirmed with _____ test on steam distillates of the material.
chromotrophic acid
TSI salmonella rxn
Alk/A H2S
qualitative type of PCR amplification
Insulated isothermal PCR (iiPCR)
convection principle
Rayleigh-Bernard
used to generate fluorescent signal when a specific sequence
of DNA of the pathogen is amplified.
Fluorogenic probe
hydrolysis chemistry
the primers and probe target
__ gene that is conserved among all Salmonella spp.,
yrfH
PCR steps
denaturation
annealing
extension
DNA primers are annealed to the ___ into the organism.
complementary DNA
Using a heat-stable DNA polymerase (e.g.
___), the primers are extended with free
____, that would result to the replication of the
starting material.
Taq polymerase, from Thermus aquaticus
deoxynucleoside triphosphates (dNTPs)
For Salmonella sp. the primers and probe target yrfH gene that is conserved among all Salmonella spp., and do not react with host genomic DNA and nucleic acid of other pathogens
true
broth used in extraction of salmonella in egg
lactose and tetrathionate
medium used for extraction of salmonella in egg
XLD/SS
what is tested in Isolation of Salmonella from egg
Salmonella polyvalent antisera.
