FISH AND EGG

Question 1

Bulging or prominent eyes, transparent cornea, black pupil

Answer 1

fresh fish

Question 2

Slightly sunken cornea, somewhat full pupil

Answer 2

grade 2

Question 3

Sunken cornea, dull and milky white pupil

Answer 3

grade 3

Question 4

Much sunken heavy yellow slime, opaque cornea

Answer 4

grade 4

Question 5

utilization of grade 2

Answer 5

bagoong, drying, smoking

Question 6

utilization of grade 4

Answer 6

rendering plant, fish meal or fertilizer

Question 7

utilization of grade 3

Answer 7

condemnation/patis manufacture

Question 8

utilization of grade 1

Answer 8

quick sale for local market

Question 9

Slight smell like rotten cabbage turnip or melon-like slight smell of ammonia

Answer 9

grade 3

Question 10

Bleaching or greenish brown discoloration, heavy

Answer 10

grade 4

Question 11

muddy, milky slime, “bloom” fades

Answer 11

grade 2

Question 12

Light slime, no discoloration; scales intact and adheres to skin, in “full bloom”

Answer 12

grade 1

Question 13

In fish intended for drying, formalin is being used to

Answer 13

get rid of worms and make the scales glow.

Question 14

Smoked fish and meat product can naturally contain formalin from the wood smoke or from artificial smoking by smearing with wood distillate (“liquid smoke”)

Answer 14

true

Question 15

preliminary test for detection of formalin

Answer 15

amidol test

Question 16

he amidol test is not specific for formaldehyde as it also produces reaction with other aldehydes. For this reason, positive reaction should be further confirmed with _____ test on steam distillates of the material.

Answer 16

chromotrophic acid

Question 17

TSI salmonella rxn

Answer 17

Alk/A H2S

Question 18

qualitative type of PCR amplification

Answer 18

Insulated isothermal PCR (iiPCR)

Question 19

convection principle

Answer 19

Rayleigh-Bernard

Question 20

used to generate fluorescent signal when a specific sequence
of DNA of the pathogen is amplified.

Answer 20

Fluorogenic probe
hydrolysis chemistry

Question 21

the primers and probe target
__ gene that is conserved among all Salmonella spp.,

Answer 21

yrfH

Question 22

PCR steps

Answer 22

denaturation
annealing
extension

Question 23

DNA primers are annealed to the ___ into the organism.

Answer 23

complementary DNA

Question 24

Using a heat-stable DNA polymerase (e.g.
___), the primers are extended with free
____, that would result to the replication of the
starting material.

Answer 24

Taq polymerase, from Thermus aquaticus

deoxynucleoside triphosphates (dNTPs)

Question 25

For Salmonella sp. the primers and probe target yrfH gene that is conserved among all Salmonella spp., and do not react with host genomic DNA and nucleic acid of other pathogens

Answer 25

true

Question 26

broth used in extraction of salmonella in egg

Answer 26

lactose and tetrathionate

Question 27

medium used for extraction of salmonella in egg

Answer 27

XLD/SS

Question 28

what is tested in Isolation of Salmonella from egg

Answer 28

Salmonella polyvalent antisera.