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HEMATOLOGY (LEMAR) > FIRST PART > Flashcards

FIRST PART Flashcards

1
Q

Total blood volume in adult

A

5-6 liters or 7-8% total body weight

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2
Q

causes of hypovolemia

A
  • lose whole blood (blood lose)
  • lose of plasma
  • dehydration
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3
Q

causes of hypervolemia

A
  • blood transfusion
  • intravenous fluid
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4
Q

liquid portion of clotted blood

A

serum

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5
Q

liquid portion of anticoagulated blood

A

plasma

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6
Q

Action of EDTA

A

prevents coagulation by chelating calcium

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7
Q

inversion EDTA

A

8-10x

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8
Q

most commonly used anticoagulant in hematology

A

EDTA

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9
Q

anticoagulant of choice of CBC

A

EDTA

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9
Q

concentration of EDTA

A

1.5 mg/mL

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10
Q

EDTA cannot be used for coagulation because

A

it interferes with Factor 5 and thrombin-fibrinogen reaction

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11
Q

liquid form EDTA

A

sequestrene

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12
Q

preparation of blood films using EDTA up to how many hours?

A

2-3 hours

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13
Q

Anticoagulant of MODIFIED WESTERGREEN ESR

A

EDTA

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14
Q

What is platelet satellitosis

A

Platelet adhere PMNS

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15
Q

Platelet encircling the peripheral borders of neutrophil

A

Platelet satellitosis

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16
Q

remedy for platelet satellitosis

A

rpt. platelet count using citrate then multiply the obtain platelet count by 1.1

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17
Q

Excessive concentration of EDTA causes

A

shrinkage of RBC leading to decreased HCT and ESR increased MCHC but normal hemoglobin
- cause degenerative changes of WBC also platelet will swell and break eventually cause falsely elevated platelet count due to broken fragments

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18
Q

Changes in blood at room temp after 3 hours

A
  1. Degenerative cellular changes
  2. WBC’s may show vacuolation of the cytoplasm , poorly defined cytoplasmic borders, irregular shape of nuclei
  3. platelet increase in size then distegrates
19
Q

Changes in blood at room temp after 6 hours

A
  1. RBC swells(increase MCV)
  2. Decrease ESR
  3. Increased OFT
19
Q

inversion: sodium citrate

19
Q

What is the action of sodium citrate

A

prevents coagulation by binding calcium of the blood in a soluble complex

19
Q

temp for refrigeration of blood

A

4 degree C

20
Q

anticoagulant and blood ration of sodium citrate

21
Q

concentration of sodium citrate

A

0.109 (3.2%) sodium citrate

22
Q

dilution: sodium citrate

22
Q

buffered 0.109 M citrate increase the stability of

A

FACTORS 5 and 8

22
Q

formula to determine the amount of sodium citrate

A

100-hematocrit over 595 -hematocrit x mL of whole blood used

23
Q

anticoagulant/blood ratio: black tube

23
Q

Orig/standard westergreen ESR

A

black tube

HEMATOLOGY (LEMAR) (2 decks)

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